Literature DB >> 3107511

Characterization of chromosome and plasmid transformation in Bacillus subtilis using gently lysed protoplasts.

T Akamatsu, J Sekiguchi.   

Abstract

Competent cells of Bacillus subtilis were transformed with DNA from gently lysed protoplasts. Significant linkages among markers separated by distances of approximately 2.3% of the total chromosome were found, which have not been detected for conventional transformation. In comparison to previous reports, enhanced plasmid transformation was observed [4.0 X 10(7) transformants per microgram DNA (one transformant per 5 X 10(4) molecules added)], when competent cells were transformed with DNA from lysed protoplasts harboring pUB110.

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Year:  1987        PMID： 3107511     DOI： 10.1007/bf00410935

Source DB:  PubMed          Journal:  Arch Microbiol        ISSN： 0302-8933            Impact factor:   2.552


  16 in total

1.  Characterization of plasmid transformation in Bacillus subtilis: kinetic properties and the effect of DNA conformation.

Authors:  S Contente; D Dubnau
Journal:  Mol Gen Genet       Date:  1979-01-02

2.  REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

Authors:  C Anagnostopoulos; J Spizizen
Journal:  J Bacteriol       Date:  1961-05       Impact factor: 3.490

3.  Genetic and enzymic studies on the recombination process in Bacillus subtilis.

Authors:  G Mazza; A Fortunato; E Ferrari; U Canosi; A Falaschi; M Polsinelli
Journal:  Mol Gen Genet       Date:  1975

4.  Transformation of Bacillus subtilis: transforming ability of deoxyribonucleic acid in lysates of L-forms or protoplasts.

Authors:  G E Bettinger; F E Young
Journal:  J Bacteriol       Date:  1975-06       Impact factor: 3.490

5.  Selection methods in bacilli for recombinants and transformants of intra- and interspecific fused protoplasts.

Authors:  T Akamatsu; J Sekiguchi
Journal:  Arch Microbiol       Date:  1983-07       Impact factor: 2.552

Review 6.  The Bacillus subtilis chromosome.

Authors:  D J Henner; J A Hoch
Journal:  Microbiol Rev       Date:  1980-03

7.  Genes affecting the productivity of alpha-amylase in Bacillus subtilis Marburg.

Authors:  J Sekiguchi; N Takada; H Okada
Journal:  J Bacteriol       Date:  1975-02       Impact factor: 3.490

8.  Gene-enzyme relationships of aromatic acid biosynthesis in Bacillus subtilis.

Authors:  J A Hoch; E W Nester
Journal:  J Bacteriol       Date:  1973-10       Impact factor: 3.490

9.  Molecular cloning of genetically active fragments of Bacillus DNA in Bacillus subtilis and properties of the vector plasmid pUB110.

Authors:  K M Keggins; P S Lovett; E J Duvall
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

10.  Molecular cloning of a thermostable neutral protease gene from Bacillus stearothermophilus in a vector plasmid and its expression in Bacillus stearothermophilus and Bacillus subtilis.

Authors:  M Fujii; M Takagi; T Imanaka; S Aiba
Journal:  J Bacteriol       Date:  1983-05       Impact factor: 3.490
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  1 in total

1.  Further studies on recombination in diploid clones from Bacillus subtilis protoplast fusion.

Authors:  C Sanchez-Rivas; C Karmazyn-Campelli; C Levi-Meyrueis
Journal:  Mol Gen Genet       Date:  1988-10
  1 in total

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