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Literature DB >> 105246

Characterization of plasmid transformation in Bacillus subtilis: kinetic properties and the effect of DNA conformation.

Abstract

Transformation of competent cells of Bacillus subtilis with antibiotic resistance plasmid DNA has shown that (a) competence for plasmid and chromosomal DNA develops with similar kinetics; (b) DNA linearized with a variety of restriction endonucleases does not transform; (c) CCC plasmid DNA is inactivated for transformation by a single nick; (d) T4 ligase restores transforming activity to both nicked and linearized DNA; (E) CCC relaxed DNA is fully active in transformation; (f) the DNA concentration-dependence of plasmid transformation is first order; and (g) plasmid transformation proceeds with a low efficiency, requiring the uptake of 10(3) to 10(4) DNA molecules per transformant. Based on this information, a model for the processing of chromosomal, plasmid and transfecting DNA is proposed.

Entities: Species

Mesh：

Substances：
DNA, Bacterial

Year: 1979 PMID： 105246 DOI： 10.1007/bf00267416

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

22 in total

1. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

Authors: C Anagnostopoulos; J Spizizen
Journal: J Bacteriol Date: 1961-05 Impact factor: 3.490

Review 2. Recombinant DNA.

Authors: R L Sinsheimer
Journal: Annu Rev Biochem Date: 1977 Impact factor: 23.643

3. General method for the isolation of plasmid deoxyribonucleic acid.

Authors: P Guerry; D J LeBlanc; S Falkow
Journal: J Bacteriol Date: 1973-11 Impact factor: 3.490

4. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors: P A Sharp; B Sugden; J Sambrook
Journal: Biochemistry Date: 1973-07-31 Impact factor: 3.162

Review 5. Restriction endonucleases.

Authors: R J Roberts
Journal: CRC Crit Rev Biochem Date: 1976-11

6. Conditions affecting the isolation from transformed cells of Bacillus subtilis of high-molecular-weight single-stranded deoxyribonucleic acid of donor origin.

Authors: R Davidoff-Abelson; D Dubnau
Journal: J Bacteriol Date: 1973-10 Impact factor: 3.490

Review 7. Transfection in B. subtilis.

Authors: T A Trautner; H C Spatz
Journal: Curr Top Microbiol Immunol Date: 1973 Impact factor: 4.291

8. Fate of transforming DNA following uptake by competent Bacillus subtilis. IV. The endwise attachment and uptake of transforming DNA.

Authors: D Dubnau; C Cirigliano
Journal: J Mol Biol Date: 1972-02-28 Impact factor: 5.469

9. Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194.

Authors: B Weisblum; M Y Graham; T Gryczan; D Dubnau
Journal: J Bacteriol Date: 1979-01 Impact factor: 3.490

10. Integration of deoxyribonuclease-treated DNA in bacillus subtilis transformation.

Authors: W F Bodmer
Journal: J Gen Physiol Date: 1966-07 Impact factor: 4.086

74 in total

1. Characterization of PBSX, a defective prophage of Bacillus subtilis.

Authors: H E Wood; M T Dawson; K M Devine; D J McConnell
Journal: J Bacteriol Date: 1990-05 Impact factor: 3.490

2. Replication properties of pIM13, a naturally occurring plasmid found in Bacillus subtilis, and of its close relative pE5, a plasmid native to Staphylococcus aureus.

Authors: S J Projan; M Monod; C S Narayanan; D Dubnau
Journal: J Bacteriol Date: 1987-11 Impact factor: 3.490

Review 8. Dissecting the effects of antibiotics on horizontal gene transfer: Analysis suggests a critical role of selection dynamics.

Authors: Allison J Lopatkin; Tatyana A Sysoeva; Lingchong You
Journal: Bioessays Date: 2016-10-04 Impact factor: 4.345

9. Biochemical and genetic characterization of the tet determinant of Bacillus plasmid pAB124.

Authors: S J Eccles; I Chopra
Journal: J Bacteriol Date: 1984-04 Impact factor: 3.490

10. Promoter-probe plasmid for Bacillus subtilis.

Authors: C E Donnelly; A L Sonenshein
Journal: J Bacteriol Date: 1984-03 Impact factor: 3.490