Literature DB >> 11337984

Purification and characterization of an extracellular lipase from Penicillium candidum.

B Ruiz1, A Farrés, E Langley, F Masso, S Sánchez.   

Abstract

Penicillium candidum produces and secretes a single extracellular lipase with a monomer molecular weight of 29 kDa. However, this enzyme forms dimers and higher molecular weight aggregates under nondenaturing conditions. The lipase from P. candidum was purified 37-fold using Octyl-Sepharose CL-4B and DEAE-Sephadex columns. The optimal assay conditions for lipase activity were 35 degrees C and pH 9. The lipase was stable in the pH range of 5-6 with a pl of 5.5, but rapid loss of the enzyme activity was observed above 25 degrees C. Tributyrin was found to be the best substrate for the P. candidum lipase, among those tested. Metal ions such as Fe2+ and Cu2+ inhibited enzymatic activity and only Ca2+ was able to slightly enhance lipase activity. Ionic detergents inhibited the activity of the enzyme, whereas nonionic detergents stimulated lipase activity.

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Year:  2001        PMID： 11337984     DOI： 10.1007/s11745-001-0719-3

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  14 in total

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Authors:  M A Taipa; M R Aires-Barros; J M Cabral
Journal:  J Biotechnol       Date:  1992-11       Impact factor: 3.307

2.  Purification and properties of lipase from Penicillium simplicissimum.

Authors:  H Sztajer; H Lünsdorf; H Erdmann; U Menge; R Schmid
Journal:  Biochim Biophys Acta       Date:  1992-03-25

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Authors:  J Savitha; C Ratledge
Journal:  World J Microbiol Biotechnol       Date:  1992-03       Impact factor: 3.312

4.  Effects of nutrients, temperature and relative humidity on germination and longevity of the ascospores of Chaetomium thermophile var. coprophile.

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Journal:  Mycologia       Date:  1971 Sep-Oct       Impact factor: 2.696

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Lipase from a Brazilian strain of Penicillium citrinum.

Authors:  M C Pimentel; N Krieger; L C Coelho; J O Fontana; E H Melo; W M Ledingham; J L Lima Filho
Journal:  Appl Biochem Biotechnol       Date:  1994-10       Impact factor: 2.926

7.  Cloning and nucleotide sequence of cDNA encoding a lipase from Fusarium heterosporum.

Authors:  T Nagao; Y Shimada; A Sugihara; Y Tominaga
Journal:  J Biochem       Date:  1994-09       Impact factor: 3.387

8.  Purification and characterization of a lipase from Aspergillus oryzae.

Authors:  J Toida; K Kondoh; M Fukuzawa; K Ohnishi; J Sekiguchi
Journal:  Biosci Biotechnol Biochem       Date:  1995-07       Impact factor: 2.043

9.  Lipase from Chromobacterium viscosum: biochemical characterization indicating homology to the lipase from Pseudomonas glumae.

Authors:  M A Taipa; K Liebeton; J V Costa; J M Cabral; K E Jaeger
Journal:  Biochim Biophys Acta       Date:  1995-06-06

10.  Purification and properties of a lipase from Penicillium expansum.

Authors:  W Stöcklein; H Sztajer; U Menge; R D Schmid
Journal:  Biochim Biophys Acta       Date:  1993-06-12
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  3 in total

1.  Purification and characterization of an extracellular lipase from Geotrichum marinum.

Authors:  Youliang Huang; Robert Locy; John D Weete
Journal:  Lipids       Date:  2004-03       Impact factor: 1.880

2.  Improving of hydrolases biosythesis by solid-state fermentation of Penicillium camemberti on rapeseed cake.

Authors:  Filip Boratyński; Ewa Szczepańska; Aleksandra Grudniewska; Radosław Gniłka; Teresa Olejniczak
Journal:  Sci Rep       Date:  2018-07-05       Impact factor: 4.379

3.  Purification, Biochemical and Kinetic Characterization of a Novel Alkaline sn-1,3-Regioselective Triacylglycerol Lipase from Penicilliumcrustosum Thom Strain P22 Isolated from Moroccan Olive Mill Wastewater.

Authors:  Ismail Hasnaoui; Ahlem Dab; Sondes Mechri; Houssam Abouloifa; Ennouamane Saalaoui; Bassem Jaouadi; Alexandre Noiriel; Abdeslam Asehraou; Abdelkarim Abousalham
Journal:  Int J Mol Sci       Date:  2022-10-07       Impact factor: 6.208
  3 in total

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