| Literature DB >> 30982661 |
Qiumei Du1, Ashley R Hoover1, Igor Dozmorov1, Prithvi Raj1, Shaheen Khan1, Erika Molina1, Tsung-Cheng Chang2, Maria Teresa de la Morena3, Ondine B Cleaver2, Joshua T Mendell4, Nicolai S C van Oers5.
Abstract
MicroRNAs (miRNAs) are processed from primary miRNA transcripts (pri-miRNAs), many of which are annotated as long noncoding RNAs (lncRNAs). We assessed whether MIR205HG, the host gene for miR-205, has independent functions as an lncRNA. Comparing mice with targeted deletions of MIR205HG and miR-205 revealed a functional role for the lncRNA in the anterior pituitary. Mice lacking MIR205HG had a temporal reduction in Pit1, growth hormone, and prolactin. This was mediated, in part, through the ability of this lncRNA to bind and regulate the transcriptional activity of Pit1 in conjunction with Zbtb20. Knockdown of MIR205HG in lactotropes decreased the expression of Pit1, Zbtb20, prolactin, and growth hormone, while its overexpression enhanced the levels of these transcripts. The effects of MIR205HG on the pituitary were independent of miR-205. The data support a role for MIR205HG as an lncRNA that regulates growth hormone and prolactin production in the anterior pituitary.Entities:
Keywords: MIR205HG; endocrine hormones; growth hormone; miR-205; pituitary gland; pituitary transcriptome; pouf1; prolactin; thymus
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Year: 2019 PMID： 30982661 PMCID： PMC9131289 DOI： 10.1016/j.devcel.2019.03.012
Source DB: PubMed Journal: Dev Cell ISSN： 1534-5807 Impact factor: 13.417