Literature DB >> 24581438

UV cross-linking of interacting RNA and protein in cultured cells.

Emi Sei1, Nicholas K Conrad2.   

Abstract

RNA-protein interactions play indispensable roles in the regulation of cellular functions. Biochemical characterization of these complexes is often done by immunoprecipitation (IP) of RNA-binding proteins (RBPs) followed by identification of co-immunoprecipitated RNAs. This protocol couples ultraviolet (UV) irradiation with IP to determine whether a specific protein interacts directly with a specific RNA in living cells.
© 2014 Elsevier Inc. All rights reserved.

Keywords:  Cross-linking protocol (CLIP); Immunoprecipitation; Northern blotting; Proteinase K treatment; RNA immunoprecipitation (RIP) and cell-mixing protocols; RNA–protein interactions; Ribonucleoprotein particles (RNPs); Sodium dodecyl sulfate (SDS) Lysis Buffer; UV cross-linking; Western blotting

Mesh：

Substances：

Year:  2014        PMID： 24581438     DOI： 10.1016/B978-0-12-420120-0.00004-9

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  8 in total

1.  Influenza Virus NS1 Protein-RNA Interactome Reveals Intron Targeting.

Authors:  Liang Zhang; Juan Wang; Raquel Muñoz-Moreno; Min Kim; Ramanavelan Sakthivel; Wei Mo; Dandan Shao; Aparna Anantharaman; Adolfo García-Sastre; Nicholas K Conrad; Beatriz M A Fontoura
Journal:  J Virol       Date:  2018-11-27       Impact factor: 5.103

2.  Analysis of HIV-1 Matrix-Envelope Cytoplasmic Tail Interactions.

Authors:  Ayna Alfadhli; August O Staubus; Philip R Tedbury; Mariia Novikova; Eric O Freed; Eric Barklis
Journal:  J Virol       Date:  2019-10-15       Impact factor: 5.103

3.  5'-UTR SNP of FGF13 causes translational defect and intellectual disability.

Authors:  Xingyu Pan; Jingrong Zhao; Zhiying Zhou; Jijun Chen; Zhenxing Yang; Yuxuan Wu; Meizhu Bai; Yang Jiao; Yun Yang; Xuye Hu; Tianling Cheng; Qianyun Lu; Bin Wang; Chang-Lin Li; Ying-Jin Lu; Lei Diao; Yan-Qing Zhong; Jing Pan; Jianmin Zhu; Hua-Sheng Xiao; Zi-Long Qiu; Jinsong Li; Zefeng Wang; Jingyi Hui; Lan Bao; Xu Zhang
Journal:  Elife       Date:  2021-06-29       Impact factor: 8.140

4.  Characterization of the Molecular Interactions That Govern the Packaging of Viral RNA Segments into Rift Valley Fever Phlebovirus Particles.

Authors:  Breanna Tercero; Krishna Narayanan; Kaori Terasaki; Shinji Makino
Journal:  J Virol       Date:  2021-06-24       Impact factor: 5.103

5.  MIR205HG Is a Long Noncoding RNA that Regulates Growth Hormone and Prolactin Production in the Anterior Pituitary.

Authors:  Qiumei Du; Ashley R Hoover; Igor Dozmorov; Prithvi Raj; Shaheen Khan; Erika Molina; Tsung-Cheng Chang; Maria Teresa de la Morena; Ondine B Cleaver; Joshua T Mendell; Nicolai S C van Oers
Journal:  Dev Cell       Date:  2019-04-11       Impact factor: 13.417

6.  NOVA1 regulates hTERT splicing and cell growth in non-small cell lung cancer.

Authors:  Andrew T Ludlow; Mandy Sze Wong; Jerome D Robin; Kimberly Batten; Laura Yuan; Tsung-Po Lai; Nicole Dahlson; Lu Zhang; Ilgen Mender; Enzo Tedone; Mohammed E Sayed; Woodring E Wright; Jerry W Shay
Journal:  Nat Commun       Date:  2018-08-06       Impact factor: 14.919

7.  Kaposi's sarcoma-associated herpesvirus ORF57 protein protects viral transcripts from specific nuclear RNA decay pathways by preventing hMTR4 recruitment.

Authors:  Julio C Ruiz; Olga V Hunter; Nicholas K Conrad
Journal:  PLoS Pathog       Date:  2019-02-20       Impact factor: 6.823

8.  A Novel Small RNA Promotes Motility and Virulence of Enterohemorrhagic Escherichia coli O157:H7 in Response to Ammonium.

Authors:  Tianyuan Jia; Bin Liu; Huiqian Mu; Chengqian Qian; Lu Wang; Linxing Li; Gege Lu; Wenxuan Zhu; Xi Guo; Bin Yang; Di Huang; Lu Feng; Bin Liu
Journal:  mBio       Date:  2021-03-09       Impact factor: 7.867
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.