Literature DB >> 30937821

Bci528I, a new isoschizomer of EcoRI isolated from Bacillus circulans 528.

Sung-Ryong Ra1, Myong-Suk Kim2, Chon-Il Paek2, Yong-Chol Pak2, Song-Hui Pak2, Hyong-Bom Pak2, Kum-Chol Ri3.   

Abstract

Bacillus circulans 528 produces a restriction endonuclease, Bci528I which is an isoschizomer of EcoRI. We purified the enzyme, using Sephadex G-150, Phospho-cellulose, DEAE-cellulose, Hepharin-Sepharose CL-6B chromatography. The specific activity of Bci528I was 29,400 U/mg·protein. Bci528I recognizes 5'-GAATTC-3' in dsDNA and cleaves between G and A of the recognition sequence, producing a symmetric four base 5'overhang.

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Year:  2019        PMID： 30937821     DOI： 10.1007/s12223-019-00694-3

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  14 in total

1.  A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.

Authors:  Richard J Roberts; Marlene Belfort; Timothy Bestor; Ashok S Bhagwat; Thomas A Bickle; Jurate Bitinaite; Robert M Blumenthal; Sergey Kh Degtyarev; David T F Dryden; Kevin Dybvig; Keith Firman; Elizaveta S Gromova; Richard I Gumport; Stephen E Halford; Stanley Hattman; Joseph Heitman; David P Hornby; Arvydas Janulaitis; Albert Jeltsch; Jytte Josephsen; Antal Kiss; Todd R Klaenhammer; Ichizo Kobayashi; Huimin Kong; Detlev H Krüger; Sanford Lacks; Martin G Marinus; Michiko Miyahara; Richard D Morgan; Noreen E Murray; Valakunja Nagaraja; Andrzej Piekarowicz; Alfred Pingoud; Elisabeth Raleigh; Desirazu N Rao; Norbert Reich; Vladimir E Repin; Eric U Selker; Pang-Chui Shaw; Daniel C Stein; Barry L Stoddard; Waclaw Szybalski; Thomas A Trautner; James L Van Etten; Jorge M B Vitor; Geoffrey G Wilson; Shuang-yong Xu
Journal:  Nucleic Acids Res       Date:  2003-04-01       Impact factor: 16.971

2.  Taxonomic specificity of restriction-modification enzymes.

Authors:  A Janulaitis; R Kazlauskiene; L Lazareviciute; R Gilvonauskaite; D Steponaviciene; M Jagelavicius; M Petrusyte; J Bitinaite; Z Vezeviciute; E Kiuduliene
Journal:  Gene       Date:  1988-12-25       Impact factor: 3.688

3.  DNA restriction enzyme from E. coli.

Authors:  M Meselson; R Yuan
Journal:  Nature       Date:  1968-03-23       Impact factor: 49.962

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Sequence analysis of the DNA encoding the Eco RI endonuclease and methylase.

Authors:  P J Greene; M Gupta; H W Boyer; W E Brown; J M Rosenberg
Journal:  J Biol Chem       Date:  1981-03-10       Impact factor: 5.157

6.  A general method for the purification of restriction enzymes.

Authors:  P J Greene; H L Heyneker; F Bolivar; R L Rodriguez; M C Betlach; A A Covarrubias; K Backman; D J Russel; R Tait; H W Boyer
Journal:  Nucleic Acids Res       Date:  1978-07       Impact factor: 16.971

7.  Nucleotide sequence of the Dpn II DNA methylase gene of Streptococcus pneumoniae and its relationship to the dam gene of Escherichia coli.

Authors:  B M Mannarelli; T S Balganesh; B Greenberg; S S Springhorn; S A Lacks
Journal:  Proc Natl Acad Sci U S A       Date:  1985-07       Impact factor: 11.205

8.  Evidence for horizontal transfer of the EcoT38I restriction-modification gene to chromosomal DNA by the P2 phage and diversity of defective P2 prophages in Escherichia coli TH38 strains.

Authors:  Keiko Kita; Hideaki Kawakami; Hiroaki Tanaka
Journal:  J Bacteriol       Date:  2003-04       Impact factor: 3.490

9.  REBASE--a database for DNA restriction and modification: enzymes, genes and genomes.

Authors:  Richard J Roberts; Tamas Vincze; Janos Posfai; Dana Macelis
Journal:  Nucleic Acids Res       Date:  2009-10-21       Impact factor: 16.971

10.  BaeI, another unusual BcgI-like restriction endonuclease.

Authors:  L E Sears; B Zhou; J M Aliotta; R D Morgan; H Kong
Journal:  Nucleic Acids Res       Date:  1996-09-15       Impact factor: 16.971
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