| Literature DB >> 3087918 |
P H Nibbering, J G Marijnen, A K Raap, P C Leijh, R van Furth.
Abstract
The nitrocellulose binding assay was used for quantitative studies on the cytochemical reactions for the three enzymes most frequently used in immunocytochemistry. The results show a linear relationship between the amount of enzyme immobilized on nitrocellulose and the amount of the enzyme reaction product. The similar course of the formation of the reaction product after DAB/H2O2 staining for peroxidase immobilized on nitrocellulose and for immunoperoxidase labeled cells indicates a linear relationship between the amount of enzyme-coupled antibodies bound to cells and the amount of enzyme reaction product. Furthermore, a mild acid treatment for the abolition of endogeneous peroxidase activity in tissues and cells applicable to immunoperoxidase staining procedures is proposed.Entities:
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Year: 1986 PMID： 3087918 DOI： 10.1007/bf00482988
Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564