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Literature DB >> 3084556

Identification of mycoplasma colonies by immunobinding.

Abstract

An immunobinding assay was developed to identify mycoplasma colonies on agar in pure and mixed cultures. Mycoplasma colonies on agar were transferred to nitrocellulose. The nitrocellulose was treated with specific rabbit antisera against mycoplasmas, peroxidase-conjugated goat anti-rabbit immunoglobulin G, 4-chloro-1-naphthol, and H2O2. Purple developed in the presence of specific reactions. The procedure was superior to epifluorescence.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1986 PMID： 3084556 PMCID： PMC362837 DOI： 10.1128/jcm.23.4.783-785.1986

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

12 in total

1. MYCOPLASMA SPECIES IDENTIFICATION BASED UPON GROWTH INHIBITION BY SPECIFIC ANTISERA.

Authors: W A CLYDE
Journal: J Immunol Date: 1964-06 Impact factor: 5.422

2. Detection of anaerobic mycoplasmas in cell cultures.

Authors: G J McGarrity; L L Coriell
Journal: In Vitro Date: 1973 Jul-Aug

3. Serological cross-reactions between "Mycoplasma genitalium" and M. pneumoniae.

Authors: K Lind
Journal: Lancet Date: 1982-11-20 Impact factor: 79.321

4. Serological comparison of bovine ureaplasmas.

Authors: M Ogata; H Kotani; K Yamamoto
Journal: Nihon Juigaku Zasshi Date: 1979-12

5. Dot-blotting--a novel screening assay for antibodies in hybridoma cultures.

Authors: J Sternberg; P Jeppesen
Journal: J Immunol Methods Date: 1983-11-11 Impact factor: 2.303

6. A dot-immunobinding assay for monoclonal and other antibodies.

Authors: R Hawkes; E Niday; J Gordon
Journal: Anal Biochem Date: 1982-01-01 Impact factor: 3.365

7. Immunogold for detection of antigen on nitrocellulose paper.

Authors: Y H Hsu
Journal: Anal Biochem Date: 1984-10 Impact factor: 3.365

8. Antigens of Mycoplasma hominis.

Authors: M B Brown; F C Minion; J K Davis; D G Pritchard; G H Cassell
Journal: Sex Transm Dis Date: 1983 Oct-Dec Impact factor: 2.830

9. Serological cross-reactions between Mycoplasma genitalium and Mycoplasma pneumoniae.

Authors: K Lind; B O Lindhardt; H J Schütten; J Blom; C Christiansen
Journal: J Clin Microbiol Date: 1984-12 Impact factor: 5.948

10. Monoclonal antibodies specific for cell culture mycoplasmas.

Authors: D W Buck; R H Kennett; G McGarrity
Journal: In Vitro Date: 1982-04

13 in total

1. Immunoglobulin class- and subclass-specific responses to Mycoplasma pulmonis in sera and secretions of naturally infected Sprague-Dawley female rats.

Authors: M B Brown; L Reyes
Journal: Infect Immun Date: 1991-06 Impact factor: 3.441

8. Variant colony surface antigenic phenotypes within mycoplasma strain populations: implications for species identification and strain standardization.

Authors: R Rosengarten; D Yogev
Journal: J Clin Microbiol Date: 1996-01 Impact factor: 5.948

9. The Vlp system of Mycoplasma hyorhinis: combinatorial expression of distinct size variant lipoproteins generating high-frequency surface antigenic variation.

Authors: R Rosengarten; K S Wise
Journal: J Bacteriol Date: 1991-08 Impact factor: 3.490

10. Detection of Mycoplasma hyopneumoniae in bronchoalveolar lavage fluids of pigs by PCR.

Authors: A K Baumeister; M Runge; M Ganter; A A Feenstra; F Delbeck; H Kirchhoff
Journal: J Clin Microbiol Date: 1998-07 Impact factor: 5.948