Literature DB >> 6644033

Dot-blotting--a novel screening assay for antibodies in hybridoma cultures.

J Sternberg, P Jeppesen.   

Abstract

A novel solid phase radioimmunoassay using nitrocellulose filters, is described. The method is of particular value in screening hybridoma cultures for monoclonal antibodies against chromosomal protein. Also it permits detection of antibodies directed against epitopes stable to SDS denaturation.

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Year:  1983        PMID： 6644033     DOI： 10.1016/0022-1759(83)90382-4

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  8 in total

1.  Type IX Ehlers-Danlos syndrome and Menkes syndrome: the decrease in lysyl oxidase activity is associated with a corresponding deficiency in the enzyme protein.

Authors:  H Kuivaniemi; L Peltonen; K I Kivirikko
Journal:  Am J Hum Genet       Date:  1985-07       Impact factor: 11.025

2.  Purification of human procollagen type III N-proteinase from placenta and preparation of antiserum.

Authors:  R Halila; L Peltonen
Journal:  Biochem J       Date:  1986-10-01       Impact factor: 3.857

3.  Antigenic homology between rat sperm tail polypeptides and Sertoli cell secretory proteins.

Authors:  M Abdullah; L L Tres; H Ueda; P C Hu; A L Kierszenbaum
Journal:  Mol Cell Biochem       Date:  1988-06       Impact factor: 3.396

4.  Identification of mycoplasma colonies by immunobinding.

Authors:  H Kotani; G J McGarrity
Journal:  J Clin Microbiol       Date:  1986-04       Impact factor: 5.948

5.  Monoclonal antibody reveals heterogeneity in human aortic intima: detection of a ganglioside antigen associated with a subpopulation of intimal cells.

Authors:  A N Orekhov; G F Kalantarov; E R Andreeva; N V Prokazova; I N Trakht; L D Bergelson; V N Smirnov
Journal:  Am J Pathol       Date:  1986-03       Impact factor: 4.307

6.  Placental growth factor negatively regulates retinal endothelial cell barrier function through suppression of glucose-6-phosphate dehydrogenase and antioxidant defense systems.

Authors:  Hu Huang; Anton Lennikov; Madhu Sudhana Saddala; David Gozal; Dennis J Grab; Abdelnaby Khalyfa; Lijuan Fan
Journal:  FASEB J       Date:  2019-10-04       Impact factor: 5.834

7.  Replication of measles virus in human lymphocytes.

Authors:  T Hyypiä; P Korkiamäki; R Vainionpää
Journal:  J Exp Med       Date:  1985-06-01       Impact factor: 14.307

Review 8.  Immunoblotting and dot blotting.

Authors:  D I Stott
Journal:  J Immunol Methods       Date:  1989-05-12       Impact factor: 2.303
  8 in total

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