Literature DB >> 6083735

Immunogold for detection of antigen on nitrocellulose paper.

Y H Hsu.   

Abstract

The application of immunogold for the detection of antigen-antibody complexes on nitrocellulose paper is described. Tobacco mosaic virus (TMV) protein, either purified or in leaf extract, was bound to the nitrocellulose paper and then exposed to rabbit anti-TMV serum. The antigen-antibody complexes were detected by gold-labeled goat anti-rabbit immunoglobulin G. The gold-labeled antibody is directly visible because of its pink color. This method can detect 1-5 pg of TMV protein, either in purified form or in the unpurified plant extract, with high specificity.

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Year:  1984        PMID： 6083735     DOI： 10.1016/0003-2697(84)90542-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  12 in total

1.  Naturally occurring human IgG antibodies to intracellular and cytoskeletal components of human platelets.

Authors:  S L Pfueller; D Logan; T T Tran; R A Bilston
Journal:  Clin Exp Immunol       Date:  1990-03       Impact factor: 4.330

2.  Mixed stimuli-responsive magnetic and gold nanoparticle system for rapid purification, enrichment, and detection of biomarkers.

Authors:  Michael A Nash; Paul Yager; Allan S Hoffman; Patrick S Stayton
Journal:  Bioconjug Chem       Date:  2010-11-11       Impact factor: 4.774

3.  Identification of mycoplasma colonies by immunobinding.

Authors:  H Kotani; G J McGarrity
Journal:  J Clin Microbiol       Date:  1986-04       Impact factor: 5.948

Review 4.  Applications of immunogold labelling in animal and plant virology.

Authors:  S Patterson; B J Verduin
Journal:  Arch Virol       Date:  1987       Impact factor: 2.574

5.  Epidermal growth factor deficiency associated with diabetes mellitus.

Authors:  S Kasayama; Y Ohba; T Oka
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

6.  Chemiluminescence detection of proteins from single cells.

Authors:  P G Gillespie; A J Hudspeth
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

7.  Dot blot assay for detection of antidiacyltrehalose antibodies in tuberculous patients.

Authors:  L Vera-Cabrera; A Rendon; M Diaz-Rodriguez; V Handzel; A Laszlo
Journal:  Clin Diagn Lab Immunol       Date:  1999-09

8.  Fast dipstick dye immunoassay for detection of immunoglobulin G (IgG) and IgM antibodies of human toxoplasmosis.

Authors:  Si Jin; Zhu Yin Chang; Xu Ming; Cao Li Min; He Wei; Liang You Sheng; Guan Xiao Hong
Journal:  Clin Diagn Lab Immunol       Date:  2005-01

9.  Immunomicroscopical localization of human preformed natural antibodies against pig tissues in xenogeneic transplantation.

Authors:  N Maggiano; F Citterio; A Evangelista; U Pozzetto; M Castagneto; A Capelli
Journal:  Histochem J       Date:  1994-07

10.  Gold nanoparticles in biology and medicine: recent advances and prospects.

Authors:  L A Dykman; N G Khlebtsov
Journal:  Acta Naturae       Date:  2011-04       Impact factor: 1.845
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