| Literature DB >> 30826165 |
Julia M Rogers1, Colin T Waters2, Tom C M Seegar3, Sanchez M Jarrett4, Amelia N Hallworth5, Stephen C Blacklow6, Martha L Bulyk7.
Abstract
Transcription factors (TFs) control gene expression by binding DNA recognition sites in genomic regulatory regions. Although most forkhead TFs recognize a canonical forkhead (FKH) motif, RYAAAYA, some forkheads recognize a completely different (FHL) motif, GACGC. Bispecific forkhead proteins recognize both motifs, but the molecular basis for bispecific DNA recognition is not understood. We present co-crystal structures of the FoxN3 DNA binding domain bound to the FKH and FHL sites, respectively. FoxN3 adopts a similar conformation to recognize both motifs, making contacts with different DNA bases using the same amino acids. However, the DNA structure is different in the two complexes. These structures reveal how a single TF binds two unrelated DNA sequences and the importance of DNA shape in the mechanism of bispecific recognition.Entities:
Keywords: DNA binding sites; DNA shape; co-crystal structures; forkhead; gene regulation; protein-DNA interactions; specificity; transcription factors
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Year: 2019 PMID： 30826165 PMCID： PMC6474805 DOI： 10.1016/j.molcel.2019.01.019
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970