| Literature DB >> 30763638 |
Sarah R C Lentz1, Pratik R Chheda2, Lisa M Oppegard1, Tyrell R Towle2, Robert J Kerns2, Hiroshi Hiasa3.
Abstract
A Mg2+-water bridge between the C-3, C-4 diketo moiety of fluoroquinolones and the conserved amino acid residues in the GyrA/ParC subunit is critical for the binding of a fluoroquinolone to a topoisomerase-DNA covalent complex. The fluoroquinolone UING-5-249 (249) can bind to the GyrB subunit through its C7-aminomethylpyrrolidine group. This interaction is responsible for enhanced activities of 249 against the wild type and quinolone-resistant mutant topoisomerases. To further evaluate the effects of the 249-GyrB interaction on fluoroquinolone activity, we examined the activities of decarboxy- and thio-249 against DNA gyrase and conducted docking studies using the structure of a gyrase-ciprofloxacin-DNA ternary complex. We found that the 249-GyrB interaction rescued the activity of thio-249 but not that of decarboxy-249. A C7-group that binds more strongly to the GyrB subunit may allow for modifications at the C-4 position, leading to a novel compound that is active against the wild type and quinolone-resistant pathogens.Entities:
Keywords: DNA gyrase; Drug design; Fluoroquinolone; Quinolone resistance; Topoisomerase
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Year: 2019 PMID： 30763638 PMCID： PMC6462232 DOI： 10.1016/j.biochi.2019.02.002
Source DB: PubMed Journal: Biochimie ISSN： 0300-9084 Impact factor: 4.079