Peter Ek1, Blenda Böttiger2, Disa Dahlman3,4,5, Karin B Hansen1, Mattias Nyman2, Anna C Nilsson1. 1. a Infectious Disease Research Unit, Department of Translational Medicine , Lund University , Malmö , Sweden. 2. b Department of Clinical Microbiology, Division of Laboratory Medicine , Lund University , Lund , Sweden. 3. c Division of Psychiatry, Department of Clinical Sciences Lund , Lund University , Lund , Sweden. 4. d Malmö Addiction Centre , Skåne University Hospital , Malmö , Sweden. 5. e Center for Primary Health Care Research, Department of Clinical Sciences , Lund University/Region Skåne , Malmö , Sweden.
Abstract
BACKGROUND: Along with the current development of molecular diagnostic methods of respiratory viruses, the bedside patient sampling techniques need to be evaluated. We here asked the question whether the addition of an oropharynx swab to the traditional nasopharynx swab might improve the diagnostic yield of multiplex PCR analysis. Ct values from the two sampling sites were compared as well as patient tolerability. METHODS: In an emergency department in Malmö, Sweden, 98 adult patients with respiratory disease were sampled both from the nasopharynx and oropharynx for virus diagnostics by PCR. RESULTS: Influenza (AH1, AH3, B), human metapneumovirus (hMPV) or respiratory syncytial virus (RSV) were detected by PCR in 58 subjects. The diagnostic yield was improved by combining nasopharyngeal and oropharyngeal sampling - a virus was detected in another 6 patients compared to traditional nasopharyngeal sampling (p = .031, McNemar's test). In 38/55 subjects viral load was higher in the nasopharynx than in the oropharynx. Self-reported discomfort was significantly lower from oropharyngeal sampling than from nasopharyngeal sampling. CONCLUSIONS: Adding an oropharynx sample to a nasopharynx sample increased the diagnostic yield of respiratory viruses. Oropharyngeal sampling was well tolerated.
BACKGROUND: Along with the current development of molecular diagnostic methods of respiratory viruses, the bedside patient sampling techniques need to be evaluated. We here asked the question whether the addition of an oropharynx swab to the traditional nasopharynx swab might improve the diagnostic yield of multiplex PCR analysis. Ct values from the two sampling sites were compared as well as patient tolerability. METHODS: In an emergency department in Malmö, Sweden, 98 adult patients with respiratory disease were sampled both from the nasopharynx and oropharynx for virus diagnostics by PCR. RESULTS: Influenza (AH1, AH3, B), human metapneumovirus (hMPV) or respiratory syncytial virus (RSV) were detected by PCR in 58 subjects. The diagnostic yield was improved by combining nasopharyngeal and oropharyngeal sampling - a virus was detected in another 6 patients compared to traditional nasopharyngeal sampling (p = .031, McNemar's test). In 38/55 subjects viral load was higher in the nasopharynx than in the oropharynx. Self-reported discomfort was significantly lower from oropharyngeal sampling than from nasopharyngeal sampling. CONCLUSIONS: Adding an oropharynx sample to a nasopharynx sample increased the diagnostic yield of respiratory viruses. Oropharyngeal sampling was well tolerated.
Authors: Brian R McMinn; Asja Korajkic; Julie Kelleher; Michael P Herrmann; Adin C Pemberton; Warish Ahmed; Eric N Villegas; Kevin Oshima Journal: Sci Total Environ Date: 2021-02-09 Impact factor: 7.963
Authors: Narasimha M Beeraka; SubbaRao V Tulimilli; Medha Karnik; Surya P Sadhu; Rajeswara Rao Pragada; Gjumrakch Aliev; SubbaRao V Madhunapantula Journal: Biomed Res Int Date: 2021-06-01 Impact factor: 3.411