Christian Johana Baños-Hernández1,2, José Eduardo Navarro-Zarza3, Richard Bucala4, Jorge Hernández-Bello1,5, Isela Parra-Rojas2, María Guadalupe Ramírez-Dueñas6, Samuel García-Arellano1,5, Luis Alexis Hernández-Palma1, Andrea Carolina Machado-Sulbarán6, José Francisco Muñoz-Valle7,8. 1. Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico. 2. Doctorado en Ciencias Biomédicas, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero, Chilpancingo de los Bravo, Guerrero, Mexico. 3. Departamento de Medicina Interna-Reumatología, Hospital General de Chilpancingo "Dr. Raymundo Abarca Alarcón", Chilpancingo de los Bravo, Guerrero, Mexico. 4. Department of Medicine/Section of Rheumatology, Yale University School of Medicine, New Haven, CT, USA. 5. Instituto Transdisciplinar de Investigación y Servicios, Universidad de Guadalajara, Zapopan, Jalisco, Mexico. 6. Laboratorio de Inmunología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico. 7. Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico. biologiamolecular@hotmail.com. 8. Instituto Transdisciplinar de Investigación y Servicios, Universidad de Guadalajara, Zapopan, Jalisco, Mexico. biologiamolecular@hotmail.com.
Abstract
INTRODUCTION: Systemic sclerosis (SSc) is a complex autoimmune disease, characterized by microvascular lesions, autoimmunity, and fibrosis. It is suggested that MIF participates in the amplification of the proinflammatory process in SSc; moreover, the promoter polymorphisms - 794 CATT5-8 (rs5844572) and - 173G>C (rs755622) in the MIF gene have been associated with an increase in MIF serum levels in several autoimmune diseases. The aim of this study was to analyze the relationship of the - 794 CATT5-8 and - 173G>C MIF polymorphisms with mRNA expression, MIF serum levels, and the Th1/Th2/Th17 cytokine profile in SSc. MATERIALS AND METHODS: A case-control study was carried out that included 50 patients with SSc and 100 control subjects (CS). Both polymorphisms were genotyped by PCR and PCR-RFLP. MIF levels were measured by ELISA kit. The cytokine profile and the MIF mRNA expression were quantified by BioPlex MagPix system and real-time PCR, respectively. RESULTS: An association between the - 794 CATT7 and - 173*C MIF alleles and the 7C haplotype with SSc susceptibility was found (p < 0.05). Also, the 7C haplotype was associated with increased MIF mRNA expression (p = 0.03) in SSc. In addition, an increase of IL-1β and IL-6 serum levels in SSc patients was found as well as a positive correlation between MIF serum levels and Th1 and Th17 cytokine profiles. CONCLUSION: The MIF 7C haplotype is a susceptibility marker for SSc in the southern Mexican population and is associated with MIF mRNA expression. Moreover, there is a positive correlation between MIF serum levels and Th1 and Th17 inflammatory response in SSc.
INTRODUCTION:Systemic sclerosis (SSc) is a complex autoimmune disease, characterized by microvascular lesions, autoimmunity, and fibrosis. It is suggested that MIF participates in the amplification of the proinflammatory process in SSc; moreover, the promoter polymorphisms - 794 CATT5-8 (rs5844572) and - 173G>C (rs755622) in the MIF gene have been associated with an increase in MIF serum levels in several autoimmune diseases. The aim of this study was to analyze the relationship of the - 794 CATT5-8 and - 173G>C MIF polymorphisms with mRNA expression, MIF serum levels, and the Th1/Th2/Th17 cytokine profile in SSc. MATERIALS AND METHODS: A case-control study was carried out that included 50 patients with SSc and 100 control subjects (CS). Both polymorphisms were genotyped by PCR and PCR-RFLP. MIF levels were measured by ELISA kit. The cytokine profile and the MIF mRNA expression were quantified by BioPlex MagPix system and real-time PCR, respectively. RESULTS: An association between the - 794 CATT7 and - 173*C MIF alleles and the 7C haplotype with SSc susceptibility was found (p < 0.05). Also, the 7C haplotype was associated with increased MIF mRNA expression (p = 0.03) in SSc. In addition, an increase of IL-1β and IL-6 serum levels in SSc patients was found as well as a positive correlation between MIF serum levels and Th1 and Th17 cytokine profiles. CONCLUSION: The MIF 7C haplotype is a susceptibility marker for SSc in the southern Mexican population and is associated with MIF mRNA expression. Moreover, there is a positive correlation between MIF serum levels and Th1 and Th17 inflammatory response in SSc.
Authors: José Alvaro Lomelí-Nieto; José Francisco Muñoz-Valle; Christian Johana Baños-Hernández; José Eduardo Navarro-Zarza; Juliana Marisol Godínez-Rubí; Samuel García-Arellano; María Guadalupe Ramírez-Dueñas; Isela Parra-Rojas; Arisbeth Villanueva-Pérez; Jorge Hernández-Bello Journal: Clin Exp Med Date: 2022-05-29 Impact factor: 3.984
Authors: Theresa H Wirtz; Philipp A Reuken; Christian Jansen; Petra Fischer; Irina Bergmann; Christina Backhaus; Christoph Emontzpohl; Johanna Reißing; Elisa F Brandt; M Teresa Koenen; Kai M Schneider; Robert Schierwagen; Maximilian J Brol; Johannes Chang; Henning W Zimmermann; Nilay Köse-Vogel; Thomas Eggermann; Ingo Kurth; Christian Stoppe; Richard Bucala; Jürgen Bernhagen; Michael Praktiknjo; Andreas Stallmach; Christian Trautwein; Jonel Trebicka; Tony Bruns; Marie-Luise Berres Journal: JHEP Rep Date: 2020-12-17