Literature DB >> 30742262

Dissociation of Tissues for Single-Cell Analysis.

Andrew S Potter1, S Steven Potter2.   

Abstract

It is now a routine to carry out single-cell RNA-Seq to define the gene expression patterns of thousands of cells, thereby revolutionizing many areas of research. Projects are underway to use these techniques to create an atlas of the expressed genes in all cell types of the human body. Here we describe cold-active protease methods for single-cell dissociation of organs and tissues that better preserve the in vivo gene expression patterns.

Entities:  

Keywords:  Immediate early response genes; Organ dissociation; Psychrophilic proteases; Reduced gene expression artifacts; Single-cell suspensions

Mesh：

Year:  2019        PMID： 30742262     DOI： 10.1007/978-1-4939-9021-4_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  The need to reassess single-cell RNA sequencing datasets: the importance of biological sample processing.

Authors:  Alex M Ascensión; Marcos J Araúzo-Bravo; Ander Izeta
Journal:  F1000Res       Date:  2021-08-06

Review 2.  Practical Considerations for Single-Cell Genomics.

Authors:  Claire Regan; Jonathan Preall
Journal:  Curr Protoc       Date:  2022-08

3.  Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing.

Authors:  Nicholas W Chavkin; Shelby Cain; Kenneth Walsh; Karen K Hirschi
Journal:  J Vis Exp       Date:  2021-10-11       Impact factor: 1.355

4.  Association between particulate matter containing EPFRs and neutrophilic asthma through AhR and Th17.

Authors:  Jeffrey N Harding; Maureen Gross; Vivek Patel; Steven Potter; Stephania A Cormier
Journal:  Respir Res       Date:  2021-10-26

5.  Single-cell transcriptomic analysis of vascular endothelial cells in zebrafish embryos.

Authors:  Suman Gurung; Nicole K Restrepo; Brendan Chestnut; Laurita Klimkaite; Saulius Sumanas
Journal:  Sci Rep       Date:  2022-07-29       Impact factor: 4.996

6.  Isolation of Highly Purified and Viable Retinal Endothelial Cells.

Authors:  Nicholas W Chavkin; Kenneth Walsh; Karen K Hirschi
Journal:  J Vasc Res       Date:  2020-10-06       Impact factor: 1.934

7.  Single-cell transcriptome analysis of the zebrafish embryonic trunk.

Authors:  Sanjeeva Metikala; Satish Casie Chetty; Saulius Sumanas
Journal:  PLoS One       Date:  2021-07-07       Impact factor: 3.240

8.  Dissociation of solid tumor tissues with cold active protease for single-cell RNA-seq minimizes conserved collagenase-associated stress responses.

Authors:  Ciara H O'Flanagan; Kieran R Campbell; Allen W Zhang; Farhia Kabeer; Jamie L P Lim; Justina Biele; Peter Eirew; Daniel Lai; Andrew McPherson; Esther Kong; Cherie Bates; Kelly Borkowski; Matt Wiens; Brittany Hewitson; James Hopkins; Jenifer Pham; Nicholas Ceglia; Richard Moore; Andrew J Mungall; Jessica N McAlpine; Sohrab P Shah; Samuel Aparicio
Journal:  Genome Biol       Date:  2019-10-17       Impact factor: 13.583

Review 9.  Single Cell Analysis in Vascular Biology.

Authors:  Nicholas W Chavkin; Karen K Hirschi
Journal:  Front Cardiovasc Med       Date:  2020-03-31
  9 in total

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