Literature DB >> 3066683

Transformation of yeast by agitation with glass beads.

M C Costanzo1, T D Fox.   

Abstract

We have found that agitation of Saccharomyces cerevisiae with glass beads and plasmid DNA using a vortex mixer results in genetic transformation of the yeast cells. This method is less efficient, but considerably more convenient, than other yeast transformation procedures. The fact that the minimal requirements for transformation are simply physical damage and the presence of DNA in an osmotically supportive environment suggests that this process may occur in nature.

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Year:  1988        PMID： 3066683      PMCID： PMC1203545     

Source DB:  PubMed          Journal:  Genetics        ISSN： 0016-6731            Impact factor:   4.562


  13 in total

1.  Expression of genes transferred into monocot and dicot plant cells by electroporation.

Authors:  M Fromm; L P Taylor; V Walbot
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205

2.  Mitochondrial transformation in yeast by bombardment with microprojectiles.

Authors:  S A Johnston; P Q Anziano; K Shark; J C Sanford; R A Butow
Journal:  Science       Date:  1988-06-10       Impact factor: 47.728

3.  A short nucleotide sequence required for regulation of HIS4 by the general control system of yeast.

Authors:  T F Donahue; R S Daves; G Lucchini; G R Fink
Journal:  Cell       Date:  1983-01       Impact factor: 41.582

4.  Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo.

Authors:  N F Neff; J H Thomas; P Grisafi; D Botstein
Journal:  Cell       Date:  1983-05       Impact factor: 41.582

5.  Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.

Authors:  H Potter; L Weir; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

6.  Molecular cloning and genetic mapping of the PET494 gene of Saccharomyces cerevisiae.

Authors:  P P Müller; T D Fox
Journal:  Mol Gen Genet       Date:  1984

7.  Transformation of intact yeast cells treated with alkali cations.

Authors:  H Ito; Y Fukuda; K Murata; A Kimura
Journal:  J Bacteriol       Date:  1983-01       Impact factor: 3.490

8.  Measurement of cytoplasmic pH in Dictyostelium discoideum by using a new method for introducing macromolecules into living cells.

Authors:  M Fechheimer; C Denny; R F Murphy; D L Taylor
Journal:  Eur J Cell Biol       Date:  1986-04       Impact factor: 4.492

9.  At least two nuclear gene products are specifically required for translation of a single yeast mitochondrial mRNA.

Authors:  M C Costanzo; E C Seaver; T D Fox
Journal:  EMBO J       Date:  1986-12-20       Impact factor: 11.598

10.  A method for incorporating macromolecules into adherent cells.

Authors:  P L McNeil; R F Murphy; F Lanni; D L Taylor
Journal:  J Cell Biol       Date:  1984-04       Impact factor: 10.539
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  22 in total

1.  High-efficiency transformation of the pathogenic yeast Candida parapsilosis.

Authors:  Julia Zemanova; Jozef Nosek; Lubomir Tomaska
Journal:  Curr Genet       Date:  2003-11-26       Impact factor: 3.886

2.  Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance.

Authors:  N Ossanna; S Mischke
Journal:  Appl Environ Microbiol       Date:  1990-10       Impact factor: 4.792

3.  High-frequency nuclear transformation of Chlamydomonas reinhardtii.

Authors:  K L Kindle
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

4.  Expression of GFP in the mitochondrial compartment using DQAsome-mediated delivery of an artificial mini-mitochondrial genome.

Authors:  Diana Lyrawati; Alan Trounson; David Cram
Journal:  Pharm Res       Date:  2011-08-11       Impact factor: 4.200

5.  High-efficiency transformation of Chlamydomonas reinhardtii by electroporation.

Authors:  K Shimogawara; S Fujiwara; A Grossman; H Usuda
Journal:  Genetics       Date:  1998-04       Impact factor: 4.562

Review 6.  Ecologically driven competence for exogenous DNA uptake in yeast.

Authors:  Petar Tomev Mitrikeski
Journal:  Curr Microbiol       Date:  2015-04-01       Impact factor: 2.188

7.  Biolistic transformation of Trichoderma harzianum and Gliocladium virens using plasmid and genomic DNA.

Authors:  M Lorito; C K Hayes; A Di Pietro; G E Harman
Journal:  Curr Genet       Date:  1993-10       Impact factor: 3.886

8.  Functional interactions among two yeast mitochondrial ribosomal proteins and an mRNA-specific translational activator.

Authors:  P Haffter; T W McMullin; T D Fox
Journal:  Genetics       Date:  1991-02       Impact factor: 4.562

9.  Ethanol improves the transformation efficiency of intact yeast cells.

Authors:  V Lauermann
Journal:  Curr Genet       Date:  1991-07       Impact factor: 3.886

Review 10.  Abiotic Gene Transfer: Rare or Rampant?

Authors:  Tadej Kotnik; James C Weaver
Journal:  J Membr Biol       Date:  2016-04-11       Impact factor: 1.843
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