Literature DB >> 9560396

High-efficiency transformation of Chlamydomonas reinhardtii by electroporation.

K Shimogawara1, S Fujiwara, A Grossman, H Usuda.   

Abstract

We have established a high-efficiency method for transforming the unicellular, green alga Chlamydomonas reinhardtii by electroporation. Electroporation of strains CC3395 and CC425, cell wall-less mutants devoid of argininosuccinate lyase (encoded by ARG7), in the presence of the plasmid pJD67 (which contains ARG7) was used to optimize conditions for the introduction of exogenous DNA. The conditions that were varied included osmolarity, temperature, concentration of exogenous DNA, voltage and capacitance. Following optimization, the maximum transformation frequency obtained was 2 x 10(5) transformants per microg of DNA; this frequency is two orders of magnitude higher than obtained with the current standard method using glass beads to introduce exogenous DNA. The electroporation procedure described in this article is of general utility, and makes it feasible to isolate genes by direct complementation of Chlamydomonas reinhardtii mutants.

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Year:  1998        PMID： 9560396      PMCID： PMC1460073     

Source DB:  PubMed          Journal:  Genetics        ISSN： 0016-6731            Impact factor:   4.562


  24 in total

1.  Direct complementation of Chlamydomonas mutants with amplified YAC DNA.

Authors:  M Vashishtha; G Segil; J L Hall
Journal:  Genomics       Date:  1996-09-15       Impact factor: 5.736

2.  High efficiency transformation of Saccharomyces cerevisiae by electroporation.

Authors:  P Manivasakam; R H Schiestl
Journal:  Nucleic Acids Res       Date:  1993-09-11       Impact factor: 16.971

3.  Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation.

Authors:  L E Brown; S L Sprecher; L R Keller
Journal:  Mol Cell Biol       Date:  1991-04       Impact factor: 4.272

4.  Transformation of Chlamydomonas reinhardtii with silicon carbide whiskers.

Authors:  T G Dunahay
Journal:  Biotechniques       Date:  1993-09       Impact factor: 1.993

5.  Further characterization of the respiratory deficient dum-1 mutation of Chlamydomonas reinhardtii and its use as a recipient for mitochondrial transformation.

Authors:  B L Randolph-Anderson; J E Boynton; N W Gillham; E H Harris; A M Johnson; M P Dorthu; R F Matagne
Journal:  Mol Gen Genet       Date:  1993-01

6.  Insertion mutagenesis of Chlamydomonas reinhardtii by electroporation and heterologous DNA.

Authors:  D K Tang; S Y Qiao; M Wu
Journal:  Biochem Mol Biol Int       Date:  1995-08

7.  Studies on transformation of Escherichia coli with plasmids.

Authors:  D Hanahan
Journal:  J Mol Biol       Date:  1983-06-05       Impact factor: 5.469

8.  Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure.

Authors:  R D Gietz; R H Schiestl; A R Willems; R A Woods
Journal:  Yeast       Date:  1995-04-15       Impact factor: 3.239

9.  Targeted disruption of chloroplast genes in Chlamydomonas reinhardtii.

Authors:  S M Newman; N W Gillham; E H Harris; A M Johnson; J E Boynton
Journal:  Mol Gen Genet       Date:  1991-11

10.  Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase.

Authors:  K L Kindle; R A Schnell; E Fernández; P A Lefebvre
Journal:  J Cell Biol       Date:  1989-12       Impact factor: 10.539
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  165 in total

1.  The Cia5 gene controls formation of the carbon concentrating mechanism in Chlamydomonas reinhardtii.

Authors:  Y Xiang; J Zhang; D P Weeks
Journal:  Proc Natl Acad Sci U S A       Date:  2001-04-17       Impact factor: 11.205

Review 2.  Plant transformation technology. Developments and applications.

Authors:  C A Newell
Journal:  Mol Biotechnol       Date:  2000-09       Impact factor: 2.695

3.  Stable chloroplast transformation in Chlamydomonas reinhardtii using microprojectile bombardment.

Authors:  M M el-Sheekh
Journal:  Folia Microbiol (Praha)       Date:  2000       Impact factor: 2.099

4.  Chlamydomonas reinhardtii genome project. A guide to the generation and use of the cDNA information.

Authors:  Jeff Shrager; Charles Hauser; Chiung-Wen Chang; Elizabeth H Harris; John Davies; Jeff McDermott; Raquel Tamse; Zhaodou Zhang; Arthur R Grossman
Journal:  Plant Physiol       Date:  2003-02       Impact factor: 8.340

5.  Plastid terminal oxidase 2 (PTOX2) is the major oxidase involved in chlororespiration in Chlamydomonas.

Authors:  Laura Houille-Vernes; Fabrice Rappaport; Francis-André Wollman; Jean Alric; Xenie Johnson
Journal:  Proc Natl Acad Sci U S A       Date:  2011-12-05       Impact factor: 11.205

Review 6.  Chlamydomonas reinhardtii at the crossroads of genomics.

Authors:  Arthur R Grossman; Elizabeth E Harris; Charles Hauser; Paul A Lefebvre; Diego Martinez; Dan Rokhsar; Jeff Shrager; Carolyn D Silflow; David Stern; Olivier Vallon; Zhaoduo Zhang
Journal:  Eukaryot Cell       Date:  2003-12

7.  A novel rhodanese is required to maintain chloroplast translation in Chlamydomonas.

Authors:  Liming Luo; David L Herrin
Journal:  Plant Mol Biol       Date:  2012-05-29       Impact factor: 4.076

8.  Haematococcus as a promising cell factory to produce recombinant pharmaceutical proteins.

Authors:  Amir Ata Saei; Parisa Ghanbari; Abolfazl Barzegari
Journal:  Mol Biol Rep       Date:  2012-06-26       Impact factor: 2.316

Review 9.  Chlamydomonas reinhardtii: a convenient model system for the study of DNA repair in photoautotrophic eukaryotes.

Authors:  Daniel Vlcek; Andrea Sevcovicová; Barbara Sviezená; Eliska Gálová; Eva Miadoková
Journal:  Curr Genet       Date:  2007-11-09       Impact factor: 3.886

10.  A type II NAD(P)H dehydrogenase mediates light-independent plastoquinone reduction in the chloroplast of Chlamydomonas.

Authors:  Frédéric Jans; Emmanuel Mignolet; Pierre-Alain Houyoux; Pierre Cardol; Bart Ghysels; Stéphan Cuiné; Laurent Cournac; Gilles Peltier; Claire Remacle; Fabrice Franck
Journal:  Proc Natl Acad Sci U S A       Date:  2008-12-12       Impact factor: 11.205
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