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Literature DB >> 16167053

Innovation: Photoactivatable fluorescent proteins.

Konstantin A Lukyanov¹, Dmitry M Chudakov, Sergey Lukyanov, Vladislav V Verkhusha.

Abstract

The fluorescence characteristics of photoactivatable proteins can be controlled by irradiating them with light of a specific wavelength, intensity and duration. This provides unique possibilities for the optical labelling and tracking of living cells, organelles and intracellular molecules in a spatio-temporal manner. Here, we discuss the properties of the available photoactivatable fluorescent proteins and their potential applications.

Mesh：

Substances：
Luminescent Proteins

Year: 2005 PMID： 16167053 DOI： 10.1038/nrm1741

Source DB: PubMed Journal: Nat Rev Mol Cell Biol ISSN： 1471-0072 Impact factor: 94.444

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Cited

140 in total

1. Three-dimensional photoactivated localization microscopy with genetically expressed probes.

Authors: Kelsey Temprine; Andrew G York; Hari Shroff
Journal: Methods Mol Biol Date: 2015

Review 2. Probing and tracking organelles in living plant cells.

Authors: Tong Chen; Xiaohua Wang; Daniel von Wangenheim; Maozhong Zheng; Jozef Šamaj; Wanquan Ji; Jinxing Lin
Journal: Protoplasma Date: 2011-12-20 Impact factor: 3.356

3. Expression, purification, crystallization and preliminary X-ray analysis of eCGP123, an extremely stable monomeric green fluorescent protein with reversible photoswitching properties.

Authors: Craig Don Paul; Daouda A K Traore; Emma Byres; Jamie Rossjohn; Rodney J Devenish; Csaba Kiss; Andrew Bradbury; Matthew C J Wilce; Mark Prescott
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Date: 2011-09-30

Review 4. Imaging intracellular protein dynamics by spinning disk confocal microscopy.

Authors: Samantha Stehbens; Hayley Pemble; Lyndsay Murrow; Torsten Wittmann
Journal: Methods Enzymol Date: 2012 Impact factor: 1.600

5. Protein-flexibility mediated coupling between photoswitching kinetics and surrounding viscosity of a photochromic fluorescent protein.

Authors: Ya-Ting Kao; Xinxin Zhu; Wei Min
Journal: Proc Natl Acad Sci U S A Date: 2012-02-10 Impact factor: 11.205

Innovation: Photoactivatable fluorescent proteins.

1. Three-dimensional photoactivated localization microscopy with genetically expressed probes.

Review 2. Probing and tracking organelles in living plant cells.

3. Expression, purification, crystallization and preliminary X-ray analysis of eCGP123, an extremely stable monomeric green fluorescent protein with reversible photoswitching properties.

Review 4. Imaging intracellular protein dynamics by spinning disk confocal microscopy.

5. Protein-flexibility mediated coupling between photoswitching kinetics and surrounding viscosity of a photochromic fluorescent protein.

6. Superresolution imaging of multiple fluorescent proteins with highly overlapping emission spectra in living cells.

Review 7. Proteins on the move: insights gained from fluorescent protein technologies.

8. Molecular basis of the light-driven switching of the photochromic fluorescent protein Padron.

9. Photochemical tools to study dynamic biological processes.

10. Analysis of ER resident proteins in Saccharomyces cerevisiae: implementation of H/KDEL retrieval sequences.