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Literature DB >> 3063400

The primary structure of the leu1+ gene of Schizosaccharomyces pombe.

Y Kikuchi¹, Y Kitazawa, H Shimatake, M Yamamoto.

Abstract

A DNA fragment which carries the leu1 gene encoding beta-isopropylmalate dehydrogenase in Schizosaccharomyces pombe has been isolated by complementation of an E. coli leuB mutation. This 1.5 kb DNA fragment complements not only the S. pombe leu1 mutation, but also the S. cerevisiae leu2 mutation. The nucleotide sequence of the essential part of the leu1 gene and its flanking regions was determined. This sequence contains an open reading frame of 371 codons, from which a protein having a Mr = 39,732 can be predicted. The deduced amino acid sequence and its codon usage were compared with those of the S. cerevisiae LEU2 protein. The cloned DNA will be a useful marker when transforming S. pombe.

Entities: Gene Species

Mesh：

Substances：

Year: 1988 PMID： 3063400 DOI： 10.1007/bf00419995

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

19 in total

1. High-frequency transformation of the fission yeast Schizosaccharomyces pombe.

Authors: D Beach; P Nurse
Journal: Nature Date: 1981-03-12 Impact factor: 49.962

2. New M13 vectors for cloning.

Authors: J Messing
Journal: Methods Enzymol Date: 1983 Impact factor: 1.600

3. Correlation between the abundance of yeast transfer RNAs and the occurrence of the respective codons in protein genes. Differences in synonymous codon choice patterns of yeast and Escherichia coli with reference to the abundance of isoaccepting transfer RNAs.

Authors: T Ikemura
Journal: J Mol Biol Date: 1982-07-15 Impact factor: 5.469

4. High guanine plus cytosine content in the third letter of codons of an extreme thermophile. DNA sequence of the isopropylmalate dehydrogenase of Thermus thermophilus.

Authors: Y Kagawa; H Nojima; N Nukiwa; M Ishizuka; T Nakajima; T Yasuhara; T Tanaka; T Oshima
Journal: J Biol Chem Date: 1984-03-10 Impact factor: 5.157

5. Transformation of yeast by a replicating hybrid plasmid.

Authors: J D Beggs
Journal: Nature Date: 1978-09-14 Impact factor: 49.962

6. DNA sequence required for efficient transcription termination in yeast.

Authors: K S Zaret; F Sherman
Journal: Cell Date: 1982-03 Impact factor: 41.582

7. Cloned ural locus of Schizosaccharomyces pombe propagates autonomously in this yeast assuming a polymeric form.

Authors: J Sakaguchi; M Yamamoto
Journal: Proc Natl Acad Sci U S A Date: 1982-12 Impact factor: 11.205

8. The Yarrowia lipolytica LEU2 gene.

Authors: L S Davidow; F S Kaczmarek; J R DeZeeuw; S W Conlon; M R Lauth; D A Pereira; A E Franke
Journal: Curr Genet Date: 1987 Impact factor: 3.886

9. Codon selection in yeast.

Authors: J L Bennetzen; B D Hall
Journal: J Biol Chem Date: 1982-03-25 Impact factor: 5.157

10. Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization.

Authors: T Uemura; M Yanagida
Journal: EMBO J Date: 1984-08 Impact factor: 11.598

19 in total

1. The Saccharomyces cerevisiae Hap5p homolog from fission yeast reveals two conserved domains that are essential for assembly of heterotetrameric CCAAT-binding factor.

Authors: D S McNabb; K A Tseng; L Guarente
Journal: Mol Cell Biol Date: 1997-12 Impact factor: 4.272

2. Mutations in a Saccharomyces cerevisiae host showing increased holding stability of the heterologous plasmid pSR1.

Authors: K Irie; H Araki; Y Oshima
Journal: Mol Gen Genet Date: 1991-02

3. Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies.

Authors: E Apolinario; M Nocero; M Jin; C S Hoffman
Journal: Curr Genet Date: 1993-12 Impact factor: 3.886

The primary structure of the leu1+ gene of Schizosaccharomyces pombe.

1. High-frequency transformation of the fission yeast Schizosaccharomyces pombe.

2. New M13 vectors for cloning.

3. Correlation between the abundance of yeast transfer RNAs and the occurrence of the respective codons in protein genes. Differences in synonymous codon choice patterns of yeast and Escherichia coli with reference to the abundance of isoaccepting transfer RNAs.

4. High guanine plus cytosine content in the third letter of codons of an extreme thermophile. DNA sequence of the isopropylmalate dehydrogenase of Thermus thermophilus.

5. Transformation of yeast by a replicating hybrid plasmid.

6. DNA sequence required for efficient transcription termination in yeast.

7. Cloned ural locus of Schizosaccharomyces pombe propagates autonomously in this yeast assuming a polymeric form.

8. The Yarrowia lipolytica LEU2 gene.

9. Codon selection in yeast.

10. Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization.

1. The Saccharomyces cerevisiae Hap5p homolog from fission yeast reveals two conserved domains that are essential for assembly of heterotetrameric CCAAT-binding factor.

2. Mutations in a Saccharomyces cerevisiae host showing increased holding stability of the heterologous plasmid pSR1.

3. Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies.

4. Stockpiling of Cdc25 during a DNA replication checkpoint arrest in Schizosaccharomyces pombe.

5. Cloning of a cDNA for rape chloroplast 3-isopropylmalate dehydrogenase by genetic complementation in yeast.

6. Cloning the Schizosaccharomyces pombe lys2+ gene and construction of new molecular genetic tools.

7. Structural analyses of DNA fragments integrated by illegitimate recombination in Schizosaccharomyces pombe.

8. Mammalian promoter element function in the fission yeast Schizosaccharomyces pombe.

Review 9. Phylogenesis of fission yeasts. Contradictions surrounding the origin of a century old genus.

Review 10. Gene database for the fission yeast Schizosaccharomyces pombe.