| Literature DB >> 30622179 |
Tina W Han1,2,3, Wenlei Ye1,2,3, Neville P Bethel4, Mario Zubia1,2,3, Andrew Kim1,2,3, Kathy H Li5, Alma L Burlingame5, Michael Grabe4, Yuh Nung Jan1,2,3, Lily Y Jan6,2,3.
Abstract
Calcium-activated phospholipid scramblase mediates the energy-independent bidirectional translocation of lipids across the bilayer, leading to transient or, in the case of apoptotic scrambling, sustained collapse of membrane asymmetry. Cells lacking TMEM16F-dependent lipid scrambling activity are deficient in generation of extracellular vesicles (EVs) that shed from the plasma membrane in a Ca2+-dependent manner, namely microvesicles. We have adapted chemical induction of giant plasma membrane vesicles (GPMVs), which require both TMEM16F-dependent phospholipid scrambling and calcium influx, as a kinetic assay to investigate the mechanism of TMEM16F activity. Using the GPMV assay, we identify and characterize both inactivating and activating mutants that elucidate the mechanism for TMEM16F activation and facilitate further investigation of TMEM16F-mediated lipid translocation and its role in extracellular vesiculation.Entities:
Keywords: GPMV; TMEM16F; calcium influx; extracellular vesicles; phospholipid scrambling
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Year: 2019 PMID： 30622179 PMCID： PMC6347726 DOI： 10.1073/pnas.1817498116
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205