Hamed Shoorei1, Arash Khaki2, Amir Afshin Khaki3, Alireza Ali Hemmati4, Maryam Moghimian5, Majid Shokoohi6. 1. Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. 2. Department of Pathology, Tabriz Branch, Islamic Azad University, Tabriz, Iran. 3. Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Obstetrics and Gynecology, Universitätsklinikum Schleswig-Holstein Campus Lübeck, Lübeck, Germany. Electronic address: dr.aakhaki@gmail.com. 4. Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Histology and Embryology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. 5. Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran. 6. Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
BACKGROUND: Diabetes is one of the most chronic and widespread diseases causing the damages to the male reproductive system. Nowadays, several studies have been performed to show the role of phenolic compounds in reducing the complications of diabetes. Carvacrol is a phenolic monoterpene which has been shown to have much therapeutic efficacy in various diseases. METHODS: Thirty-two adult male Wistar rats (n = 8 in each group) were used in this experimental study. The induction of diabetes was performed using a single intraperitoneal (IP) injection of STZ (50 mg/kg). Rats were assigned into the following groups: control group, diabetic group, diabetic group daily fed with carvacrol at a dose of 75 mg/kg for 8 weeks, and the control group daily fed with carvacrol at a dose of 75 mg/kg for 8 weeks. RESULTS: Treatment with carvacrol significantly improved the histological morphology of the testis, reduced the tissue activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzymes, and diminished the elevated levels of tissue malondialdehyde (MDA) (p < 0.05). Moreover, our results showed that carvacrol significantly decreased Bax and increased Bcl-2 at the levels of gene and protein expression. It also significantly (p < 0.05) reduced the rate of germ cell apoptosis. CONCLUSION: It seems that the treatment with carvacrol mitigates testicular tissue damage in diabetic rats possibly through its antioxidant properties.
BACKGROUND:Diabetes is one of the most chronic and widespread diseases causing the damages to the male reproductive system. Nowadays, several studies have been performed to show the role of phenolic compounds in reducing the complications of diabetes. Carvacrol is a phenolic monoterpene which has been shown to have much therapeutic efficacy in various diseases. METHODS: Thirty-two adult male Wistar rats (n = 8 in each group) were used in this experimental study. The induction of diabetes was performed using a single intraperitoneal (IP) injection of STZ (50 mg/kg). Rats were assigned into the following groups: control group, diabetic group, diabetic group daily fed with carvacrol at a dose of 75 mg/kg for 8 weeks, and the control group daily fed with carvacrol at a dose of 75 mg/kg for 8 weeks. RESULTS: Treatment with carvacrol significantly improved the histological morphology of the testis, reduced the tissue activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzymes, and diminished the elevated levels of tissue malondialdehyde (MDA) (p < 0.05). Moreover, our results showed that carvacrol significantly decreased Bax and increased Bcl-2 at the levels of gene and protein expression. It also significantly (p < 0.05) reduced the rate of germ cell apoptosis. CONCLUSION: It seems that the treatment with carvacrol mitigates testicular tissue damage in diabeticrats possibly through its antioxidant properties.
Authors: Hamed Shoorei; Majid Banimohammad; Maziar M Kebria; Mohammad Afshar; Mohammad Mh Taheri; Majid Shokoohi; Mohammad Sg Farashah; Mina Eftekharzadeh; Omid Akhiani; Robert Gaspar; Hamidreza Pazoki-Toroudi Journal: Exp Biol Med (Maywood) Date: 2019-02-19
Authors: Toyin Dorcas Alabi; Charon de Villiers; Stephan S du Plessis; Thomas K Monsees; Nicole L Brooks; Oluwafemi Omoniyi Oguntibeju Journal: Diabetes Metab Syndr Obes Date: 2020-11-23 Impact factor: 3.168