Hamid Heidari 1 , Maasoume Abdollahi 2 , Sima Khani 3 , Fatemeh Nojavan 4 , Samira Khani 5 Show Affiliations »
Abstract
PURPOSE: Infertility is one of the systemic problems in diabetic men. The purpose of the present study is investigation of the effects of the Alpinia officinarum (AO) hydro-alcoholic extract on the reproductive system damages in diabetic male rats. METHODS: Twenty four male rats were randomly assigned into 4 groups (n = 6); i.e., control, diabetic control, and diabetic rats treated orally with AO extract (200 and 500 mg kg-1). A single dose (60 mg kg-1) of streptozotocin (STZ) was injected intraperitoneally (IP) to induce diabetes. After 8 weeks of treatment, blood samples, testis, and cauda epididymis were excised to evaluate specific hormonal changes, sperm parameters, and testis morphology. RESULTS: Diabetic control rats showed remarkably lower body and testicular weights, testicular volumes, and sperm parameters compared with the control group (p <0.05). Diabetic control rats also exhibited significantly decreased serum testosterone and follicle stimulating hormone (FSH). Sperm parameters were considerably enhanced in diabetic animals gavaged with AO extract. Testosterone levels were significantly elevated by administrating 500 mg kg-1 AO extract to the diabetic control rats (p <0.05). The morphological assessment of testis of treatment group (500 mg kg-1) indicated remarkable differences (p <0.05) by increasing the seminiferous tubules diameter (STD) and thickness of the seminiferous epithelium (TSE) compared with diabetic control rats. CONCLUSION: As demonstrated by the results, AO extract ameliorated sperm damage and improved sperm morphology besides improving histological damage in the testis in diabetic rats. In addition, the dose of 500 mg kg-1 worked more efficiently than 200 mg kg-1. © Springer Nature Switzerland AG 2021.
PURPOSE: Infertility is one of the systemic problems in diabetic men. The purpose of the present study is investigation of the effects of the Alpinia officinarum (AO) hydro-alcoholic extract on the reproductive system damages in diabetic male rats. METHODS: Twenty four male rats were randomly assigned into 4 groups (n = 6); i.e., control, diabetic control, and diabetic rats treated orally with AO extract (200 and 500 mg kg-1). A single dose (60 mg kg-1) of streptozotocin (STZ) was injected intraperitoneally (IP) to induce diabetes. After 8 weeks of treatment, blood samples, testis, and cauda epididymis were excised to evaluate specific hormonal changes, sperm parameters, and testis morphology. RESULTS: Diabetic control rats showed remarkably lower body and testicular weights, testicular volumes, and sperm parameters compared with the control group (p <0.05). Diabetic control rats also exhibited significantly decreased serum testosterone and follicle stimulating hormone (FSH). Sperm parameters were considerably enhanced in diabetic animals gavaged with AO extract. Testosterone levels were significantly elevated by administrating 500 mg kg-1 AO extract to the diabetic control rats (p <0.05). The morphological assessment of testis of treatment group (500 mg kg-1) indicated remarkable differences (p <0.05) by increasing the seminiferous tubules diameter (STD) and thickness of the seminiferous epithelium (TSE) compared with diabetic control rats. CONCLUSION: As demonstrated by the results, AO extract ameliorated sperm damage and improved sperm morphology besides improving histological damage in the testis in diabetic rats. In addition, the dose of 500 mg kg-1 worked more efficiently than 200 mg kg-1. © Springer Nature Switzerland AG 2021.
Entities: Chemical
Keywords:
Alpinia officinarum; Diabetic; Reproductive; Sperm; Streptozotocin
Year: 2021
PMID： 34222060 PMCID： PMC8212207 DOI： 10.1007/s40200-020-00711-0
Source DB: PubMed Journal: J Diabetes Metab Disord ISSN： 2251-6581