Emmanouil Sifnaios1, George Mastorakos2, Katerina Psarra3, Nikolaos-Dimitrios Panagopoulos4, Konstantinos Panoulis4, Nikolaos Vitoratos4, Demetrios Rizos5, George Creatsas4. 1. 2nd Department of Obstetrics and Gynecology, Medical School, Aretaieion University Hospital, National and Kapodistrian University of Athens, Athens, Greece sifneosm@gmail.com. 2. Endocrine Unit, Medical School, Aretaieion University Hospital, National and Kapodistrian University of Athens, Athens, Greece. 3. Department of Immunology - Histocompatibility, Evangelismos Hospital, Athens, Greece. 4. 2nd Department of Obstetrics and Gynecology, Medical School, Aretaieion University Hospital, National and Kapodistrian University of Athens, Athens, Greece. 5. Hormone Laboratory, Medical School, Aretaieion University Hospital, National and Kapodistrian University of Athens, Athens, Greece.
Abstract
BACKGROUND/AIM: Gestational diabetes mellitus (GDM) is a common pregnancy complication, characterized by insulin resistance and low-grade systemic inflammation with a pro-inflammatory immune system response. Our objective was to study the peripheral Th1, Th2, Th17 and Treg response in GDM compared to normal pregnancy. MATERIALS AND METHODS: Th1, Th2, Th17 and Treg subsets was determined by flow cytometry based on staining for specific intracellular cytokines, as well as C-reactive protein (CRP) and total IgE circulating levels. The health status of all offspring was also assessed 6 months post-delivery. RESULTS: A total of 49 Caucasian adult pregnant women were enrolled into a GDM (n=26) and Control (n=23) group. At the third trimester of pregnancy, the GDM group had a higher proportion of Th2, Th17 and Treg cells compared to control. Contrary to the control group, the GDM group exhibited no significant change in the Th1/Th2/Th17/Treg profile postpartum. Furthermore, higher circulating CRP and total IgE levels were noted in the GDM group compared to controls. At the 6-month post-delivery assessment, 30.8% of the offspring from the GDM group were found to have developed atopic dermatitis, food allergy or allergic proctocolitis compared to none from the control group. CONCLUSION: Compared to an uncomplicated pregnancy, GDM exhibits a significantly different peripheral T-cell profile at the third pregnancy trimester characterized by higher proportion of Th2, Th17 and Treg cells which persist six months post-delivery, while the increased high sensitivity CRP (hsCRP) levels stressed the low-grade inflammatory profile of this disease. Copyright
BACKGROUND/AIM: Gestational diabetes mellitus (GDM) is a common pregnancy complication, characterized by insulin resistance and low-grade systemic inflammation with a pro-inflammatory immune system response. Our objective was to study the peripheral Th1, Th2, Th17 and Treg response in GDM compared to normal pregnancy. MATERIALS AND METHODS:Th1, Th2, Th17 and Treg subsets was determined by flow cytometry based on staining for specific intracellular cytokines, as well as C-reactive protein (CRP) and total IgE circulating levels. The health status of all offspring was also assessed 6 months post-delivery. RESULTS: A total of 49 Caucasian adult pregnant women were enrolled into a GDM (n=26) and Control (n=23) group. At the third trimester of pregnancy, the GDM group had a higher proportion of Th2, Th17 and Treg cells compared to control. Contrary to the control group, the GDM group exhibited no significant change in the Th1/Th2/Th17/Treg profile postpartum. Furthermore, higher circulating CRP and total IgE levels were noted in the GDM group compared to controls. At the 6-month post-delivery assessment, 30.8% of the offspring from the GDM group were found to have developed atopic dermatitis, food allergy or allergic proctocolitis compared to none from the control group. CONCLUSION: Compared to an uncomplicated pregnancy, GDM exhibits a significantly different peripheral T-cell profile at the third pregnancy trimester characterized by higher proportion of Th2, Th17 and Treg cells which persist six months post-delivery, while the increased high sensitivity CRP (hsCRP) levels stressed the low-grade inflammatory profile of this disease. Copyright
Authors: Elizabeth Torres; Katelynn B Zumpf; Jody D Ciolino; Crystal T Clark; Dorothy K Sit; Emily S Miller; Katherine L Wisner Journal: Arch Womens Ment Health Date: 2022-03-22 Impact factor: 3.633
Authors: Emanuelly Bernardes-Oliveira; Daniel Lucas Dantas de Freitas; Camilo de Lelis Medeiros de Morais; Maria da Conceição de Mesquita Cornetta; Juliana Dantas de Araújo Santos Camargo; Kassio Michell Gomes de Lima; Janaina Cristiana de Oliveira Crispim Journal: Sci Rep Date: 2020-11-06 Impact factor: 4.379