| Literature DB >> 3055178 |
L Gutmann1, M D Kitzis, D Billot-Klein, F Goldstein, G Tran Van Nhieu, T Lu, J Carlet, E Collatz, R Williamson.
Abstract
TEM-7, a novel TEM-type beta-lactamase (pI 5.41) encoded on a plasmid of approximately 85 kilobases, was found in a clinical isolate of Citrobacter freundii. Strains containing this enzyme exhibited decreased susceptibility to ceftazidime (64-fold) and aztreonam (16-fold) but not to other third-generation cephalosporins. Addition of a beta-lactamase inhibitor--clavulanic acid, sulbactam, or YTR 830--restored normal susceptibility to associated compounds such as ampicillin, piperacillin, ceftazidime, and aztreonam. DNA-DNA hybridization of an intragenic probe of TEM-1 occurred with a 19-kilobase EcoRI fragment of the plasmid encoding TEM-7. A TEM-2 derivative, TEM-201, with characteristics similar to those of TEM-7 was selected spontaneously in the presence of ceftazidime in vitro.Entities:
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Year: 1988 PMID： 3055178 DOI： 10.1093/clinids/10.4.860
Source DB: PubMed Journal: Rev Infect Dis ISSN： 0162-0886