Muhammad Ab Shabbir1, Qin Wu2, Muhammad Z Shabbir3, Abdul Sajid1,4, Saeed Ahmed1, Adeel Sattar5, Yanping Tang1, Jun Li1, Muhammad K Maan1, Haihong Hao1,2, Zonghui Yuan1,2. 1. China MOA Laboratory for Risk Assessment of Quality & Safety of Livestock & Poultry Products, Huazhong Agricultural University, Wuhan 430070, PR China. 2. National Reference Laboratory of Veterinary Drug Residues & MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, PR China. 3. Quality Operation Laboratory at University of Veterinary & Animal Sciences, Lahore, 54600, Pakistan. 4. College of Veterinary Sciences & Animal Husbandry, Abdul Wali Khan University, Mardan, 23200, Pakistan. 5. Department of Pharmacology & Toxicology, University of Veterinary & Animal Sciences, Lahore, 54600, Pakistan.
Abstract
AIM: The purpose of current study is to find out relationship between cas9 gene and antimicrobial resistance in Campylobacter jejuni NCTC11168. MATERIALS & METHODS: The involvement of the cas9 gene in antimicrobial resistance of C. jejuni was determined by assessment of minimum inhibitory concentration, clustered regularly interspaced short palindromic repeats (CRISPR)-cas gene expression in standard strains, in vitro resistance development and transcriptome analysis of a cas9 deletion mutant and wild strains. RESULTS: Increased expression of CRISPR-related genes was observed in standard strains. We also observed that Δcas9 mutant strain is more sensitive to antibiotics than its wild strain. Transcriptome analysis revealed that cas9 gene regulate several genes to promote antimicrobial resistance in C. jejuni. CONCLUSION: CRISPR-cas system plays role in the enhancement of antimicrobial resistance in C. jejuni.
AIM: The purpose of current study is to find out relationship between cas9 gene and antimicrobial resistance in Campylobacter jejuni NCTC11168. MATERIALS & METHODS: The involvement of the cas9 gene in antimicrobial resistance of C. jejuni was determined by assessment of minimum inhibitory concentration, clustered regularly interspaced short palindromic repeats (CRISPR)-cas gene expression in standard strains, in vitro resistance development and transcriptome analysis of a cas9 deletion mutant and wild strains. RESULTS: Increased expression of CRISPR-related genes was observed in standard strains. We also observed that Δcas9 mutant strain is more sensitive to antibiotics than its wild strain. Transcriptome analysis revealed that cas9 gene regulate several genes to promote antimicrobial resistance in C. jejuni. CONCLUSION: CRISPR-cas system plays role in the enhancement of antimicrobial resistance in C. jejuni.
Authors: Muhammad Zulqarnain Haider; Muhammad Abu Bakr Shabbir; Tahir Yaqub; Adeel Sattar; Muhammad Kashif Maan; Sammina Mahmood; Tahir Mehmood; Hassaan Bin Aslam Journal: Biomed Res Int Date: 2022-03-28 Impact factor: 3.411