Peng He1, Guang-Lei Chen1, Shuo Li2,3, Jun Wang1, Yun-Feng Ma1, Yu-Feng Pan1, Ming He1. 1. State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, P. R. China. 2. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, P. R. China. 3. Institute of Life Sciences, Jiangsu University, Zhenjiang, P. R. China.
Abstract
BACKGROUND: The white-backed planthopper (WBPH) Sogatella furcifera, the brown planthopper (BPH) Nilaparvata lugens, and the small brown planthopper (SBPH) Laodelphax striatellus are three notorious rice pests that cause annual losses in rice yield through sap-sucking and virus transmission. Odorant-binding proteins (OBPs) are crucial olfactory genes involved in host-seeking behavior. RESULTS: We discovered the presence of 12, 12, and 16 OBPs in WBPH, BPH, and SBPH, respectively, including two novel OBPs in BPH and seven novel OBPs in SBPH. Phylogenetic analysis indicated that most of these OBPs have homologous genes, and one group (SfurOBP11, NlugOBP8, and LstrOBP2) show a slower evolution rate and are more conserved. Further, in vitro binding studies demonstrated that the three OBPs have similar binding affinities for some rice plant volatiles. Finally, RNA interference (RNAi) successfully inhibited the mRNA expression of the three OBPs, and in vivo behavioral tests showed that the OBP-deficient rice planthoppers were partly anosmic and lost some of their ability to locate rice plants. CONCLUSION: These results demonstrate the crucial role of the rice planthopper OBP genes in seeking rice plants. This information complements the current genetic resources for the development of RNAi-based transgenic rice and other pest management technologies.
BACKGROUND: The white-backed planthopper (WBPH) Sogatella furcifera, the brown planthopper (BPH) Nilaparvata lugens, and the small brown planthopper (SBPH) Laodelphax striatellus are three notorious rice pests that cause annual losses in rice yield through sap-sucking and virus transmission. Odorant-binding proteins (OBPs) are crucial olfactory genes involved in host-seeking behavior. RESULTS: We discovered the presence of 12, 12, and 16 OBPs in WBPH, BPH, and SBPH, respectively, including two novel OBPs in BPH and seven novel OBPs in SBPH. Phylogenetic analysis indicated that most of these OBPs have homologous genes, and one group (SfurOBP11, NlugOBP8, and LstrOBP2) show a slower evolution rate and are more conserved. Further, in vitro binding studies demonstrated that the three OBPs have similar binding affinities for some rice plant volatiles. Finally, RNA interference (RNAi) successfully inhibited the mRNA expression of the three OBPs, and in vivo behavioral tests showed that the OBP-deficientrice planthoppers were partly anosmic and lost some of their ability to locate rice plants. CONCLUSION: These results demonstrate the crucial role of the rice planthopper OBP genes in seeking rice plants. This information complements the current genetic resources for the development of RNAi-based transgenic rice and other pest management technologies.
Authors: Juan P Wulff; Diego F Segura; Francisco Devescovi; Irina Muntaabski; Fabian H Milla; Alejandra C Scannapieco; Jorge L Cladera; Silvia B Lanzavecchia Journal: PLoS One Date: 2021-06-17 Impact factor: 3.240