Literature DB >> 3047139

Human interleukin 1 beta is not secreted from hamster fibroblasts when expressed constitutively from a transfected cDNA.

P R Young1, D J Hazuda, P L Simon.   

Abstract

To understand the secretion and processing of interleukin-1 (IL-1), a Chinese hamster fibroblast cell line (R1610) was transfected with a human IL-1 beta cDNA under the control of the SV40 early promoter and linked to the gene for neomycin resistance. After selecting for transfected cells resistant to G418, two clones were found to constitutively express the IL-1 beta 31-kD precursor which was almost exclusively located in the cytosol. Pulse-chase experiments failed to show any secretion of IL-1 and very little IL-1 activity was detectable in cell supernatants. Furthermore, surface membrane IL-1 activity could not be detected, although low levels of activity could be released upon brief trypsin treatment. Therefore, unlike monocytes, these fibroblast cells lack the mechanism for secreting and processing of IL-1 beta.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3047139      PMCID： PMC2115202          DOI： 10.1083/jcb.107.2.447

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  46 in total

1.  Transformation of mammalian cells with genes from procaryotes and eucaryotes.

Authors:  M Wigler; R Sweet; G K Sim; B Wold; A Pellicer; E Lacy; T Maniatis; S Silverstein; R Axel
Journal:  Cell       Date:  1979-04       Impact factor: 41.582

2.  Galactokinase mutants of Chinese hamster somatic cells resistant to 2-deoxygalactose.

Authors:  J P Thirion; D Banville; H Noel
Journal:  Genetics       Date:  1976-05       Impact factor: 4.562

3.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

4.  Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

Authors:  G M Wahl; M Stern; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

5.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors:  P S Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

8.  Isolation and cell-free translation of human interferon mRNA from fibroblasts and leukocytes.

Authors:  R McCandliss; A Sloma; S Pestka
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600

9.  Epidermal cell (keratinocyte)-derived thymocyte-activating factor (ETAF).

Authors:  T A Luger; B M Stadler; S I Katz; J J Oppenheim
Journal:  J Immunol       Date:  1981-10       Impact factor: 5.422

10.  The kinetics of interleukin 1 secretion from activated monocytes. Differences between interleukin 1 alpha and interleukin 1 beta.

Authors:  D J Hazuda; J C Lee; P R Young
Journal:  J Biol Chem       Date:  1988-06-15       Impact factor: 5.157
View more
  17 in total

1.  Mechanisms of cytokine production by fibroblasts-implications for normal connective tissue homeostasis and pathological conditions.

Authors:  R N Apte
Journal:  Folia Microbiol (Praha)       Date:  1995       Impact factor: 2.099

2.  Identification of a monocyte specific pre-interleukin 1 beta convertase activity.

Authors:  M J Kostura; M J Tocci; G Limjuco; J Chin; P Cameron; A G Hillman; N A Chartrain; J A Schmidt
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205

3.  Fibroblast interleukin 1 beta: synergistic stimulation by recombinant interleukin 1 and tumor necrosis factor and posttranscriptional regulation.

Authors:  J A Elias; M M Reynolds; R M Kotloff; J A Kern
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205

4.  Interleukin-1beta and signaling of interleukin-1 in vascular wall and circulating cells modulates the extent of neointima formation in mice.

Authors:  Janet Chamberlain; David Evans; Andrea King; Rachael Dewberry; Steven Dower; David Crossman; Sheila Francis
Journal:  Am J Pathol       Date:  2006-04       Impact factor: 4.307

5.  Caspase-1 regulates Escherichia coli sepsis and splenic B cell apoptosis independently of interleukin-1beta and interleukin-18.

Authors:  Anasuya Sarkar; Mark W Hall; Matthew Exline; Judy Hart; Nina Knatz; Na Tosha Gatson; Mark D Wewers
Journal:  Am J Respir Crit Care Med       Date:  2006-08-14       Impact factor: 21.405

6.  Interleukin 1 is processed and released during apoptosis.

Authors:  K A Hogquist; M A Nett; E R Unanue; D D Chaplin
Journal:  Proc Natl Acad Sci U S A       Date:  1991-10-01       Impact factor: 11.205

7.  Pyridinyl imidazoles inhibit IL-1 and TNF production at the protein level.

Authors:  P Young; P McDonnell; D Dunnington; A Hand; J Laydon; J Lee
Journal:  Agents Actions       Date:  1993

8.  Transthyretin is an inhibitor of monocyte and endothelial cell interleukin-1 production.

Authors:  L Borish; M S King; J J Mascali; S Johnson; B Coll; L J Rosenwasser
Journal:  Inflammation       Date:  1992-10       Impact factor: 4.092

9.  Construction and expression in tumor cells of a recombinant vaccinia virus encoding human interleukin-1 beta.

Authors:  G R Peplinski; K Tsung; E D Whitman; J B Meko; J A Norton
Journal:  Ann Surg Oncol       Date:  1995-03       Impact factor: 5.344

10.  Reduction of inflammation and pyrexia in the rat by oral administration of SDZ 224-015, an inhibitor of the interleukin-1 beta converting enzyme.

Authors:  P R Elford; R Heng; L Révész; A R MacKenzie
Journal:  Br J Pharmacol       Date:  1995-06       Impact factor: 8.739
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.