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Literature DB >> 1269916

Galactokinase mutants of Chinese hamster somatic cells resistant to 2-deoxygalactose.

Abstract

The growth of Chinese hamster somatic cells was inhibited by 0.2 mg/cc of 2-deoxygalactose. Mutants partially or fully resistant to 2-deoxygalactose were isolated in a single-step or two-step selection. Some of them did not grow as well as the wild type; one of them which lacked galactokinase(EC.2.7.1.6) activity did not grow at all in galactose medium. The galactokinase kinetic properties (Vmax & kmax of the other mutants and of the wild type were different. Therefore resistance resulted either from the possible absence of galactokinase synthesis or from a structural mutation, possible a missence mutation, in the galactokinase gene.- A simple diagnostic test for juvenile cataract is proposed.

Entities: CellLine Chemical Disease Species

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Year: 1976 PMID： 1269916 PMCID： PMC1213496

Source DB: PubMed Journal: Genetics ISSN： 0016-6731 Impact factor: 4.562

8 in total

1. Selection of chinese hamster somatic cell mutants after irradiation of BUdR-labelled cells.

Authors: J P Thirion; R Labrecque; T P Vu
Journal: J Cell Physiol Date: 1976-01 Impact factor: 6.384

2. Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids.

Authors: J McCann; N E Spingarn; J Kobori; B N Ames
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

3. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

Review 4. Galactose metabolism and its regulation.

Authors: R M Cohn; S Segal
Journal: Metabolism Date: 1973-04 Impact factor: 8.694

5. Isolation of ouabain-resistant human diploid fibroblasts.

Authors: R Mankovitz; M Buchwald; R M Baker
Journal: Cell Date: 1974-11 Impact factor: 41.582

6. Effect of ploidy and mutagens on bromodeoxyuridine resistance in haploid and diploid frog cells.

Authors: L Mezger-Freed
Journal: Nat New Biol Date: 1972-02-23

7. Positive selection of mutants with deletions of the gal-chl region of the Salmonella chromosome as a screening procedure for mutagens that cause deletions.

Authors: M D Alper; B N Ames
Journal: J Bacteriol Date: 1975-01 Impact factor: 3.490

8. Genetics of somatic mammalian cells: biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism.

Authors: D Patterson; F T Kao; T T Puck
Journal: Proc Natl Acad Sci U S A Date: 1974-05 Impact factor: 11.205

8 in total

24 in total

1. Alterations in the pre-mRNA topology of the bovine growth hormone polyadenylation region decrease poly(A) site efficiency.

Authors: E R Gimmi; M E Reff; I C Deckman
Journal: Nucleic Acids Res Date: 1989-09-12 Impact factor: 16.971

2. Deletions in the SV40 late polyadenylation region downstream of the AATAAA mediate similar effects on expression in various mammalian cell lines.

Authors: E R Gimmi; K J Soprano; M Rosenberg; M E Reff
Journal: Nucleic Acids Res Date: 1988-09-26 Impact factor: 16.971

9. Toxicity of 2-deoxygalactose to Saccharomyces cerevisiae cells constitutively synthesizing galactose-metabolizing enzymes.

Authors: T Platt
Journal: Mol Cell Biol Date: 1984-05 Impact factor: 4.272

10. Efficient expression of Escherichia coli galactokinase gene in mammalian cells.

Authors: D Schümperli; B H Howard; M Rosenberg
Journal: Proc Natl Acad Sci U S A Date: 1982-01 Impact factor: 11.205

Galactokinase mutants of Chinese hamster somatic cells resistant to 2-deoxygalactose.

1. Selection of chinese hamster somatic cell mutants after irradiation of BUdR-labelled cells.

2. Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids.

3. Protein measurement with the Folin phenol reagent.

Review 4. Galactose metabolism and its regulation.

5. Isolation of ouabain-resistant human diploid fibroblasts.

6. Effect of ploidy and mutagens on bromodeoxyuridine resistance in haploid and diploid frog cells.

7. Positive selection of mutants with deletions of the gal-chl region of the Salmonella chromosome as a screening procedure for mutagens that cause deletions.

8. Genetics of somatic mammalian cells: biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism.

1. Alterations in the pre-mRNA topology of the bovine growth hormone polyadenylation region decrease poly(A) site efficiency.

2. Deletions in the SV40 late polyadenylation region downstream of the AATAAA mediate similar effects on expression in various mammalian cell lines.

3. Transcriptional activity of avian retroviral long terminal repeats directly correlates with enhancer activity.

4. Functional analysis of the transcription control region located within the avian retroviral long terminal repeat.

5. A coordinate relationship between the GALK and the TK1 genes of the Chinese hamster.

6. Affecting gene expression by altering the length and sequence of the 5' leader.

7. Promoter dependence of enhancer activity.

8. Adenovirus-induced mutations at the hypoxanthine phosphoribosyltransferase locus of Chinese hamster cells.

9. Toxicity of 2-deoxygalactose to Saccharomyces cerevisiae cells constitutively synthesizing galactose-metabolizing enzymes.

10. Efficient expression of Escherichia coli galactokinase gene in mammalian cells.