| Literature DB >> 30441031 |
John Choi, Vasily Goncharov, Jessica Kleinbart, Amy Orsborn, Bijan Pesaran.
Abstract
The size and curvature of the macaque brain present challenges for two photon laser scanning microscopy (2P-LSM). General access to the cortex requires 5-axis positioning over a range of motion wider than existing designs offer. In addition, movement artifacts due to physiological pulsations and bodily movement present particular challenges. We present a microscope and implant platform that allows for repeatable, motorized positioning and stable imaging at any point on the dorsal convexity of macaque cortex. While testing the system to image neurons expressing fluorescent proteins in an awake macaque, motion artifacts were limited to several microns.Entities:
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Year: 2018 PMID： 30441031 PMCID： PMC7414427 DOI： 10.1109/EMBC.2018.8512994
Source DB: PubMed Journal: Annu Int Conf IEEE Eng Med Biol Soc ISSN： 2375-7477