Literature DB >> 3041413

Antibody to sigma 32 cross-reacts with DnaK: association of DnaK protein with Escherichia coli RNA polymerase.

S Skelly1, C F Fu, B Dalie, B Redfield, T Coleman, N Brot, H Weissbach.   

Abstract

A polyclonal antibody to sigma 32, the heat shock sigma factor, has been used to show the presence of low levels of sigma 32 in Escherichia coli RNA polymerase preparations (E sigma 70), which explains the observed in vitro activity of E sigma 70 towards heat shock genes. The sigma 32 antibody cross-reacts with DnaK, and DnaK has been found associated with purified preparations of both E sigma 70 and the heat shock RNA polymerase, E sigma 32.

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Year:  1988        PMID： 3041413      PMCID： PMC281784          DOI： 10.1073/pnas.85.15.5497

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  25 in total

1.  Purification and properties of a soluble factor required for the deoxyribonucleic acid-directed in vitro synthesis of beta-galactosidase.

Authors:  H Kung; C Spears; H Weissbach
Journal:  J Biol Chem       Date:  1975-02-25       Impact factor: 5.157

2.  DNA-directed in vitro synthesis of proteins involved in bacterial transcription and translation.

Authors:  T Zarucki-Schulz; C Jerez; G Goldberg; H F Kung; K H Huang; N Brot; H Weissbach
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205

3.  A novel adenosine triphosphatase isolated from RNA polymerase preparations of Escherichia coli. I. Copurification and separation.

Authors:  A Ishihama; T Ikeuchi; T Yura
Journal:  J Biochem       Date:  1976-05       Impact factor: 3.387

4.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  Isolation and characterization of a temperature-sensitive dnaK mutant of Escherichia coli B.

Authors:  H Itikawa; J Ryu
Journal:  J Bacteriol       Date:  1979-05       Impact factor: 3.490

6.  A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.

Authors:  R R Burgess
Journal:  J Biol Chem       Date:  1969-11-25       Impact factor: 5.157

7.  A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.

Authors:  R R Burgess; J J Jendrisak
Journal:  Biochemistry       Date:  1975-10-21       Impact factor: 3.162

Review 8.  The genetics and regulation of heat-shock proteins.

Authors:  F C Neidhardt; R A VanBogelen; V Vaughn
Journal:  Annu Rev Genet       Date:  1984       Impact factor: 16.830

9.  Positive regulatory gene for temperature-controlled proteins in Escherichia coli.

Authors:  F C Neidhardt; R A VanBogelen
Journal:  Biochem Biophys Res Commun       Date:  1981-05-29       Impact factor: 3.575

10.  DNA-directed in vitro synthesis of beta-galactosidase. Studies with purified factors.

Authors:  H F Kung; B Redfield; B V Treadwell; B Eskin; C Spears; H Weissbach
Journal:  J Biol Chem       Date:  1977-10-10       Impact factor: 5.157
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  9 in total

Review 1.  Roles and regulation of the heat shock sigma factor sigma 32 in Escherichia coli.

Authors:  T Yura; Y Kawasaki; N Kusukawa; H Nagai; C Wada; R Yano
Journal:  Antonie Van Leeuwenhoek       Date:  1990-10       Impact factor: 2.271

2.  Role of Escherichia coli heat shock proteins DnaK and HtpG (C62.5) in response to nutritional deprivation.

Authors:  J Spence; A Cegielska; C Georgopoulos
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

3.  Hsp70 proteins, similar to Escherichia coli DnaK, in chloroplasts and mitochondria of Euglena gracilis.

Authors:  D Amir-Shapira; T Leustek; B Dalie; H Weissbach; N Brot
Journal:  Proc Natl Acad Sci U S A       Date:  1990-03       Impact factor: 11.205

4.  A member of the Hsp70 family is localized in mitochondria and resembles Escherichia coli DnaK.

Authors:  T Leustek; B Dalie; D Amir-Shapira; N Brot; H Weissbach
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

5.  The DnaK chaperone modulates the heat shock response of Escherichia coli by binding to the sigma 32 transcription factor.

Authors:  K Liberek; T P Galitski; M Zylicz; C Georgopoulos
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-15       Impact factor: 11.205

6.  A novel sigma factor is involved in expression of the rpoH gene of Escherichia coli.

Authors:  Q P Wang; J M Kaguni
Journal:  J Bacteriol       Date:  1989-08       Impact factor: 3.490

7.  Heat shock protein synthesis of the cyanobacterium Synechocystis PCC 6803: purification of the GroEL-related chaperonin.

Authors:  C Lehel; H Wada; E Kovács; Z Török; Z Gombos; I Horváth; N Murata; L Vigh
Journal:  Plant Mol Biol       Date:  1992-01       Impact factor: 4.076

8.  Tracking bacterial DNA replication forks in vivo by pulsed field gel electrophoresis.

Authors:  M Ohki; C L Smith
Journal:  Nucleic Acids Res       Date:  1989-05-11       Impact factor: 16.971

9.  DnaK, DnaJ and GrpE form a cellular chaperone machinery capable of repairing heat-induced protein damage.

Authors:  H Schröder; T Langer; F U Hartl; B Bukau
Journal:  EMBO J       Date:  1993-11       Impact factor: 11.598
  9 in total

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