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Literature DB >> 2657661

Tracking bacterial DNA replication forks in vivo by pulsed field gel electrophoresis.

Abstract

The location of chromosomal DNA replication forks was identified in synchronously replicating E. coli cultures by pulse labeling DNA at specific times with 14C-thymidine and following incorporation of radionucleotide into genomic Not I restriction fragments. This technique could be used to characterize chromosomal DNA replication, to characterize mutations which affect this process, to identify the location of DNA replication origins and termini as well as aid in the construction of macrorestriction maps. Here, we further characterize the DNA replication mutations divE and dnaK and preliminary characterize the genomic organization of E. coli isolate 15.

Entities: Chemical Species

Mesh：

Substances：
DNA, Bacterial
Thymidine

Year: 1989 PMID： 2657661 PMCID： PMC317790 DOI： 10.1093/nar/17.9.3479

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

34 in total

1. RNA terminating within the E. coli origin of replication: stringent regulation and control by DnaA protein.

Authors: L A Rokeach; J W Zyskind
Journal: Cell Date: 1986-08-29 Impact factor: 41.582

2. The terminus region of the Escherichia coli chromosome contains two separate loci that exhibit polar inhibition of replication.

Authors: T M Hill; J M Henson; P L Kuempel
Journal: Proc Natl Acad Sci U S A Date: 1987-04 Impact factor: 11.205

3. Fine structure genetic map and complementation analysis of mutations in the dnaA gene of Escherichia coli.

Authors: E B Hansen; T Atlung; F G Hansen; O Skovgaard; K von Meyenburg
Journal: Mol Gen Genet Date: 1984

4. New techniques for purifying large DNAs and studying their properties and packaging.

Authors: D C Schwartz; W Saffran; J Welsh; R Haas; M Goldenberg; C R Cantor
Journal: Cold Spring Harb Symp Quant Biol Date: 1983

5. Enzymatic replication of the origin of the Escherichia coli chromosome.

Authors: R S Fuller; J M Kaguni; A Kornberg
Journal: Proc Natl Acad Sci U S A Date: 1981-12 Impact factor: 11.205

6. New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of beta-galactosidase.

Authors: S K Shapira; J Chou; F V Richaud; M J Casadaban
Journal: Gene Date: 1983-11 Impact factor: 3.688

7. The B66.0 protein of Escherichia coli is the product of the dnaK+ gene.

Authors: C Georgopoulos; K Tilly; D Drahos; R Hendrix
Journal: J Bacteriol Date: 1982-03 Impact factor: 3.490

8. Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product.

Authors: M Ohki; F Tamura; S Nishimura; H Uchida
Journal: J Biol Chem Date: 1986-02-05 Impact factor: 5.157

9. Extensive unwinding of the plasmid template during staged enzymatic initiation of DNA replication from the origin of the Escherichia coli chromosome.

Authors: T A Baker; K Sekimizu; B E Funnell; A Kornberg
Journal: Cell Date: 1986-04-11 Impact factor: 41.582

4. Functional evidence that the principal DNA replication origin of the Streptomyces coelicolor chromosome is close to the dnaA-gyrB region.

Authors: M S Musialowski; F Flett; G B Scott; G Hobbs; C P Smith; S G Oliver
Journal: J Bacteriol Date: 1994-08 Impact factor: 3.490

Tracking bacterial DNA replication forks in vivo by pulsed field gel electrophoresis.

1. RNA terminating within the E. coli origin of replication: stringent regulation and control by DnaA protein.

2. The terminus region of the Escherichia coli chromosome contains two separate loci that exhibit polar inhibition of replication.

3. Fine structure genetic map and complementation analysis of mutations in the dnaA gene of Escherichia coli.

4. New techniques for purifying large DNAs and studying their properties and packaging.

5. Enzymatic replication of the origin of the Escherichia coli chromosome.

6. New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of beta-galactosidase.

7. The B66.0 protein of Escherichia coli is the product of the dnaK+ gene.

8. Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product.

9. Extensive unwinding of the plasmid template during staged enzymatic initiation of DNA replication from the origin of the Escherichia coli chromosome.

10. The E. coli divE mutation, which differentially inhibits synthesis of certain proteins, is in tRNASer1.

1. Physical analysis and mapping of the Mycoplasma pneumoniae chromosome.

Review 2. New approaches for physical mapping of small genomes.

3. Determination of the size of the Azotobacter vinelandii chromosome.

4. Functional evidence that the principal DNA replication origin of the Streptomyces coelicolor chromosome is close to the dnaA-gyrB region.

Review 5. Template-switching during replication fork repair in bacteria.

6. Localizing the replication origin region on the physical map of the Mycoplasma capricolum genome.

7. Suppression of the serT42 mutation with modified tRNA(1Ser) and tRNA(5Ser) genes.