Literature DB >> 3041055

Mapping of the gene coding for Epstein-Barr virus-determined nuclear antigen EBNA3 and its transient overexpression in a human cell line by using an adenovirus expression vector.

I Joab, D T Rowe, M Bodescot, J C Nicolas, P J Farrell, M Perricaudet.   

Abstract

The open reading frame which lies within the Epstein-Barr virus (EBV) T2 cDNA isolated by Bodescot et al. (M. Bodescot, O. Brison, and M. Perricaudet, Nucleic Acids Res. 14:2611-2620, 1986) was inserted into a eucaryotic expression vector containing a strong adenovirus promoter. The T2 cDNA contains viral genomic sequences from the short BLRF3 open reading frame fused to the adjacent BERF1 long open reading frame. After transfection of human cells, the recombinant plasmid directed the expression of a 140-kilodalton protein. The expressed protein had the same molecular weight, subcellular localization, and immunological characteristics as the EBV-determined nuclear antigen EBNA3, which is made in lymphocytes latently infected with EBV. Immunoprecipitation of extracts of transfected cells labeled with [32P]phosphoric acid showed that the EBNA3 protein is phosphorylated.

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Year:  1987        PMID： 3041055      PMCID： PMC255922          DOI： 10.1128/JVI.61.10.3340-3344.1987

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  28 in total

1.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

2.  DNA sequence and expression of the B95-8 Epstein-Barr virus genome.

Authors:  R Baer; A T Bankier; M D Biggin; P L Deininger; P J Farrell; T J Gibson; G Hatfull; G S Hudson; S C Satchwell; C Séguin
Journal:  Nature       Date:  1984 Jul 19-25       Impact factor: 49.962

3.  Host-range mutants of adenovirus type 5 defective for growth in HeLa cells.

Authors:  T Harrison; F Graham; J Williams
Journal:  Virology       Date:  1977-03       Impact factor: 3.616

4.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

Authors:  W N Burnette
Journal:  Anal Biochem       Date:  1981-04       Impact factor: 3.365

5.  A cis-acting element from the Epstein-Barr viral genome that permits stable replication of recombinant plasmids in latently infected cells.

Authors:  J Yates; N Warren; D Reisman; B Sugden
Journal:  Proc Natl Acad Sci U S A       Date:  1984-06       Impact factor: 11.205

6.  Stable expression in mouse cells of nuclear neoantigen after transfer of a 3.4-megadalton cloned fragment of Epstein-Barr virus DNA.

Authors:  W P Summers; E A Grogan; D Shedd; M Robert; C R Liu; G Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

7.  Epstein-Barr virus RNA. V. Viral RNA in a restringently infected, growth-transformed cell line.

Authors:  W King; A L Thomas-Powell; N Raab-Traub; M Hawke; E Kieff
Journal:  J Virol       Date:  1980-11       Impact factor: 5.103

8.  One of two Epstein-Barr virus nuclear antigens contains a glycine-alanine copolymer domain.

Authors:  K Hennessy; E Kieff
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

9.  U2 region of Epstein-Barr virus DNA may encode Epstein-Barr nuclear antigen 2.

Authors:  T Dambaugh; K Hennessy; L Chamnankit; E Kieff
Journal:  Proc Natl Acad Sci U S A       Date:  1984-12       Impact factor: 11.205

10.  Identification of Epstein-Barr virus sequences that encode a nuclear antigen expressed in latently infected lymphocytes.

Authors:  J C Hearing; J C Nicolas; A J Levine
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205
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  25 in total

1.  Epstein-Barr virus EBNA3C represses Cp, the major promoter for EBNA expression, but has no effect on the promoter of the cell gene CD21.

Authors:  S A Radkov; M Bain; P J Farrell; M West; M Rowe; M J Allday
Journal:  J Virol       Date:  1997-11       Impact factor: 5.103

Review 2.  Molecular basis for Epstein-Barr virus induced pathogenesis and disease.

Authors:  C Sample; E Kieff
Journal:  Springer Semin Immunopathol       Date:  1991

3.  cDNA cloning and transient expression of the Epstein-Barr virus-determined nuclear antigen EBNA3B in human cells and identification of novel transcripts from its coding region.

Authors:  B Kerdiles; D Walls; H Triki; M Perricaudet; I Joab
Journal:  J Virol       Date:  1990-04       Impact factor: 5.103

4.  Transcription of the Epstein-Barr virus genome during latency in growth-transformed lymphocytes.

Authors:  J Sample; E Kieff
Journal:  J Virol       Date:  1990-04       Impact factor: 5.103

5.  In vitro transcriptional activation, dimerization, and DNA-binding specificity of the Epstein-Barr virus Zta protein.

Authors:  P M Lieberman; A J Berk
Journal:  J Virol       Date:  1990-06       Impact factor: 5.103

6.  A conserved domain of the Epstein-Barr virus nuclear antigens 3A and 3C binds to a discrete domain of Jkappa.

Authors:  B Zhao; D R Marshall; C E Sample
Journal:  J Virol       Date:  1996-07       Impact factor: 5.103

7.  Responsiveness of the Epstein-Barr virus NotI repeat promoter to the Z transactivator is mediated in a cell-type-specific manner by two independent signal regions.

Authors:  P M Lieberman; J M Hardwick; S D Hayward
Journal:  J Virol       Date:  1989-07       Impact factor: 5.103

8.  The spliced BZLF1 gene of Epstein-Barr virus (EBV) transactivates an early EBV promoter and induces the virus productive cycle.

Authors:  C M Rooney; D T Rowe; T Ragot; P J Farrell
Journal:  J Virol       Date:  1989-07       Impact factor: 5.103

Review 9.  Human herpesviruses: a consideration of the latent state.

Authors:  J G Stevens
Journal:  Microbiol Rev       Date:  1989-09

10.  Distinction between Epstein-Barr virus type A (EBNA 2A) and type B (EBNA 2B) isolates extends to the EBNA 3 family of nuclear proteins.

Authors:  M Rowe; L S Young; K Cadwallader; L Petti; E Kieff; A B Rickinson
Journal:  J Virol       Date:  1989-03       Impact factor: 5.103
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