Literature DB >> 3038941

An assessment of the sensitivity of three methods for the detection of rotavirus.

A L Martin, E A Follett.   

Abstract

A comparison was made of the sensitivity of a current commercial ELISA for detecting rotavirus in faecal specimens with the more complex, technically demanding systems of electron microscopy and polyacrylamide gel electrophoresis. Even after modification, the ELISA failed to detect 22% of specimens with particles identifiable by electron microscopy. Polyacrylamide gel electrophoresis failed to identify 2 out of 50 specimens with particles present but did distinguish 2 group C rotaviruses.

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Year:  1987        PMID： 3038941     DOI： 10.1016/0166-0934(87)90029-2

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  5 in total

1.  Evaluation of two enzyme immunoassays for detection of human rotaviruses in fecal specimens.

Authors:  B R Eing; G May; H G Baumeister; J E Kühn
Journal:  J Clin Microbiol       Date:  2001-12       Impact factor: 5.948

2.  Enzyme linked immunosorbent assay for detecting adenoviruses in stool specimens: comparison with electron microscopy and isolation.

Authors:  A L Martin; G Kudesia
Journal:  J Clin Pathol       Date:  1990-06       Impact factor: 3.411

3.  Comparison of a new rapid test (TestPack Rotavirus) with standard enzyme immunoassay and electron microscopy for the detection of rotavirus in symptomatic hospitalized children.

Authors:  R G Brooks; L Brown; R B Franklin
Journal:  J Clin Microbiol       Date:  1989-04       Impact factor: 5.948

4.  Proportional morbidity rates of enteropathogens among diarrheic dairy calves in central Spain.

Authors:  R de la Fuente; A García; J A Ruiz-Santa-Quiteria; M Luzón; D Cid; S García; J A Orden; M Gómez-Bautista
Journal:  Prev Vet Med       Date:  1998-08-07       Impact factor: 2.670

5.  Survey of rotavirus infection in a dairy herd: comparison between polyacrylamide gel electrophoresis and two commercial tests.

Authors:  J García-Sánchez; C Corral; N G Halaihel; M C Simon; J L Alonso; J L Muzquiz; C Ortega; O Girones
Journal:  Vet Microbiol       Date:  1993-04       Impact factor: 3.293
  5 in total

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