Literature DB >> 11724877

Evaluation of two enzyme immunoassays for detection of human rotaviruses in fecal specimens.

B R Eing1, G May, H G Baumeister, J E Kühn.   

Abstract

The two assays evaluated in this study (the Ridascreen rotavirus and the Pathfinder rotavirus) exhibited comparable sensitivities (100%) but highly divergent positive predictive values (93.74 and 57.7%, respectively) when compared on 393 specimens. This difference should be considered when using these tests on collectives with an unknown or low prevalence.

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Year:  2001        PMID: 11724877      PMCID: PMC88581          DOI: 10.1128/JCM.39.12.4532-4534.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  18 in total

1.  Comparison of electron microscopy, enzyme-linked immunosorbent assay, solid-phase radioimmunoassay, and indirect immunofluorescence for detection of human rotavirus antigen in faeces.

Authors:  C J Birch; N I Lehmann; A J Hawker; J A Marshall; I D Gust
Journal:  J Clin Pathol       Date:  1979-07       Impact factor: 3.411

2.  Detection of human rotavirus by nucleic acid analysis in comparison to enzyme-linked immunoassay and electron microscopy.

Authors:  B Selb; H G Baumeister; G Maass; H W Doerr
Journal:  Eur J Clin Microbiol       Date:  1985-02       Impact factor: 3.267

3.  Detection of rotavirus by a latex agglutination test, Rotalex: comparison with electron microscopy, immunofluorescence, polyacrylamide gel electrophoresis, and enzyme linked immunosorbent assay.

Authors:  R B Moosai; R Alcock; T M Bell; F R Laidler; J S Peiris; A P Wyn-Jones; C R Madeley
Journal:  J Clin Pathol       Date:  1985-06       Impact factor: 3.411

4.  Detection of rotavirus in faecal specimens by enzyme immunoassay, latex agglutination and electron microscopy.

Authors:  I Julkunen; J Savolainen; A Hautanen; T Hovi
Journal:  Scand J Infect Dis       Date:  1985

5.  Comparison of direct and indirect enzyme immunoassays with direct ultracentrifugation before electron microscopy for detection of rotaviruses.

Authors:  G W Hammond; G S Ahluwalia; F G Barker; G Horsman; P R Hazelton
Journal:  J Clin Microbiol       Date:  1982-07       Impact factor: 5.948

6.  Enzyme-linked immunosorbent assays based on polyclonal and monoclonal antibodies for rotavirus detection.

Authors:  G M Beards; A D Campbell; N R Cottrell; J S Peiris; N Rees; R C Sanders; J A Shirley; H C Wood; T H Flewett
Journal:  J Clin Microbiol       Date:  1984-02       Impact factor: 5.948

7.  Evaluation of a commercial enzyme immunoassay kit for rotavirus detection.

Authors:  B H Keswick; T W Hejkal; H L DuPont; L K Pickering
Journal:  Diagn Microbiol Infect Dis       Date:  1983-06       Impact factor: 2.803

8.  Comparison of solid-phase immune electron microscopy by use of protein A with direct electron microscopy and enzyme-linked immunosorbent assay for detection of rotavirus in stool.

Authors:  L Svensson; M Grandien; C A Pettersson
Journal:  J Clin Microbiol       Date:  1983-11       Impact factor: 5.948

9.  Comparison of six methods for detecting human rotavirus in stools.

Authors:  F Morinet; F Ferchal; R Colimon; Y Pérol
Journal:  Eur J Clin Microbiol       Date:  1984-04       Impact factor: 3.267

10.  Comparison of direct electron microscopy, immune electron microscopy, and rotavirus enzyme-linked immunosorbent assay for detection of gastroenteritis viruses in children.

Authors:  C D Brandt; H W Kim; W J Rodriguez; L Thomas; R H Yolken; J O Arrobio; A Z Kapikian; R H Parrott; R M Chanock
Journal:  J Clin Microbiol       Date:  1981-05       Impact factor: 5.948

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  5 in total

1.  Detection and genotyping of human rotavirus VP4 and VP7 genes by reverse transcriptase PCR and reverse hybridization.

Authors:  Leen-Jan van Doorn; Bernhard Kleter; Evert Hoefnagel; Isabelle Stainier; Annick Poliszczak; Brigitte Colau; Wim Quint
Journal:  J Clin Microbiol       Date:  2009-06-24       Impact factor: 5.948

2.  Detection of Rotavirus in children with acute gastroenteritis in Zagazig University Hospitals in Egypt.

Authors:  Salwa Badrelsabbah Ibrahim; Abdallah Abdelkader El-Bialy; Mervat Soliman Mohammed; Azza Omar El-Sheikh; Ahmed Elhewala; Shereen Bahgat
Journal:  Electron Physician       Date:  2015-09-16

3.  Rotavirus antigenemia as a common event among children hospitalised for severe, acute gastroenteritis in Belém, northern Brazil.

Authors:  Maria Cleonice A Justino; Erika A Campos; Joana D'arc P Mascarenhas; Luana S Soares; Sylvia de Fátima S Guerra; Ismari P Furlaneto; Manoel Jaime C Pavão; Tassio S Maciel; Fredison P Farias; Orvácio M Bezerra; Caio Breno G Vinente; Rodrigo José S Barros; Alexandre C Linhares
Journal:  BMC Pediatr       Date:  2019-06-12       Impact factor: 2.125

4.  Genetic characterization of two G8P[8] rotavirus strains isolated in Guangzhou, China, in 2020/21: evidence of genome reassortment.

Authors:  Si-Jie Wang; Li-Na Chen; Song-Mei Wang; Hong-Lu Zhou; Chao Qiu; Baoming Jiang; Tian-Yi Qiu; Sheng-Li Chen; Lorenz von Seidlein; Xuan-Yi Wang
Journal:  BMC Infect Dis       Date:  2022-06-28       Impact factor: 3.667

5.  New diagnostic biomarker in acute diarrhea due to bacterial infection in children.

Authors:  Hassan M Al-Asy; Rasha M Gamal; Ahmed M Abd Albaset; Mohammed G Elsanosy; Maali M Mabrouk
Journal:  Int J Pediatr Adolesc Med       Date:  2017-02-13
  5 in total

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