Literature DB >> 3037539

Functional analysis of a retroviral host-range mutant: altered long terminal repeat sequences allow expression in embryonal carcinoma cells.

F Hilberg, C Stocking, W Ostertag, M Grez.   

Abstract

A retroviral host-range neomycin-resistant myeloproliferative sarcoma virus mutant, which is expressed in the embryonal carcinoma cell lines F9 and PCC4aza1R, was molecularly cloned and analyzed. This mutant virus, PCMV, differs from myeloproliferative sarcoma virus by two major deletions, one of which spans exactly a 75-base-pair repeat of the long terminal repeat. Functional analysis of recombinant viruses shows that the host-range expansion of PCMV is a property of nucleotide changes within the U3 region of the long terminal repeat. Furthermore, expression assays of chimeric long terminal repeats show that the enhancer region of PCMV joined to the promoter region of Moloney murine leukemia virus is sufficient to direct the synthesis of chloramphenicol acetyltransferase in F9 and PCC4 cells.

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Year:  1987        PMID： 3037539      PMCID： PMC298829          DOI： 10.1073/pnas.84.15.5232

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  39 in total

1.  Long terminal repeat sequences impart hematopoietic transformation properties to the myeloproliferative sarcoma virus.

Authors:  C Stocking; R Kollek; U Bergholz; W Ostertag
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205

2.  Negative regulation of viral enhancers in undifferentiated embryonic stem cells.

Authors:  C M Gorman; P W Rigby; D P Lane
Journal:  Cell       Date:  1985-09       Impact factor: 41.582

3.  Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus.

Authors:  R Mann; R C Mulligan; D Baltimore
Journal:  Cell       Date:  1983-05       Impact factor: 41.582

4.  Common control of the heat shock gene and early adenovirus genes: evidence for a cellular E1A-like activity.

Authors:  M J Imperiale; H T Kao; L T Feldman; J R Nevins; S Strickland
Journal:  Mol Cell Biol       Date:  1984-05       Impact factor: 4.272

5.  Integration-specific retrovirus expression in embryonal carcinoma cells.

Authors:  J Sorge; A E Cutting; V D Erdman; J W Gautsch
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

6.  Isolation of polyomavirus mutants multiadapted to murine embryonal carcinoma cells.

Authors:  F Melin; H Pinon; C Kress; D Blangy
Journal:  J Virol       Date:  1985-03       Impact factor: 5.103

7.  Isolation and characterization of polyoma virus mutants able to develop in embryonal carcinoma cells.

Authors:  M Vasseur; C Kress; N Montreau; D Blangy
Journal:  Proc Natl Acad Sci U S A       Date:  1980-02       Impact factor: 11.205

8.  Resistance of teratocarcinoma stem cells to infection with simian virus 40: early events.

Authors:  D E Swartzendruber; T D Friedrich; J M Lehman
Journal:  J Cell Physiol       Date:  1977-10       Impact factor: 6.384

9.  Isolation of embryonal carcinoma cell lines that express integrated recombinant genes flanked by the Moloney murine leukemia virus long terminal repeat.

Authors:  M Taketo; E Gilboa; M I Sherman
Journal:  Proc Natl Acad Sci U S A       Date:  1985-04       Impact factor: 11.205

10.  Common features of polyomavirus mutants selected on PCC4 embryonal carcinoma cells.

Authors:  F Melin; H Pinon; C Reiss; C Kress; N Montreau; D Blangy
Journal:  EMBO J       Date:  1985-07       Impact factor: 11.598
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  38 in total

1.  Lack of shielding of primer binding site silencer-mediated repression of an internal promoter in a retrovirus vector by the putative insulators scs, BEAD-1, and HS4.

Authors:  C Modin; F S Pedersen; M Duch
Journal:  J Virol       Date:  2000-12       Impact factor: 5.103

2.  Analysis of the binding proteins and activity of the long terminal repeat of Moloney murine leukemia virus during differentiation of mouse embryonal carcinoma cells.

Authors:  T Tsukiyama; O Niwa; K Yokoro
Journal:  J Virol       Date:  1991-06       Impact factor: 5.103

3.  Functional heterogeneity of a large family of human LTR-like promoters and enhancers.

Authors:  A Feuchter; D Mager
Journal:  Nucleic Acids Res       Date:  1990-03-11       Impact factor: 16.971

4.  Sequences and factors required for the F9 embryonal carcinoma stem cell E1a-like activity.

Authors:  E J Murray; D Stott; P W Rigby
Journal:  Mol Cell Biol       Date:  1991-11       Impact factor: 4.272

Review 5.  Host restriction factors blocking retroviral replication.

Authors:  Daniel Wolf; Stephen P Goff
Journal:  Annu Rev Genet       Date:  2008       Impact factor: 16.830

6.  Two blocks in Moloney murine leukemia virus expression in undifferentiated F9 embryonal carcinoma cells as determined by transient expression assays.

Authors:  G Feuer; M Taketo; R C Hanecak; H Fan
Journal:  J Virol       Date:  1989-05       Impact factor: 5.103

7.  Development of a retroviral vector for inducible expression of transforming growth factor beta 1.

Authors:  M L McGeady; P M Arthur; M Seidman
Journal:  J Virol       Date:  1990-07       Impact factor: 5.103

8.  Derivatives of Moloney murine sarcoma virus capable of being transcribed in embryonal carcinoma stem cells have gained a functional Sp1 binding site.

Authors:  V E Prince; P W Rigby
Journal:  J Virol       Date:  1991-04       Impact factor: 5.103

9.  Genetically modified skin fibroblasts persist long after transplantation but gradually inactivate introduced genes.

Authors:  T D Palmer; G J Rosman; W R Osborne; A D Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1991-02-15       Impact factor: 11.205

10.  Embryonal long terminal repeat-binding protein is a murine homolog of FTZ-F1, a member of the steroid receptor superfamily.

Authors:  T Tsukiyama; H Ueda; S Hirose; O Niwa
Journal:  Mol Cell Biol       Date:  1992-03       Impact factor: 4.272
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