Literature DB >> 2352331

Development of a retroviral vector for inducible expression of transforming growth factor beta 1.

M L McGeady1, P M Arthur, M Seidman.   

Abstract

A retroviral vector system for the expression of exogenous genes under the control of an inducible promoter was developed. By utilizing this system, the cDNA for human transforming growth factor beta 1 (TGF-beta 1) was inserted into a retroviral vector under the control of an internal mouse metallothionein promoter and introduced via infection into normal rat kidney fibroblasts (NRK-49F) and epithelial cells (NRK-52E), Chinese hamster ovary cells (CHO), and the human monocytic cell line U937. Control of TGF-beta 1 expression, achieved by Cd2+ induction of vector-encoded TGF-beta 1 mRNA, was cell line specific and resulted in a concomitant increase in neutralizable TGF-beta 1 production by the cells. Autocrine stimulation of vector-containing cells by vector-encoded TGF-beta 1 was detected by an increase in soft-agar colony formation of NRK-49F infectants compared with that of the control cells. In addition, the use of a second internal promoter in a retroviral vector of similar design allowed isolation of stable infectants from a cell line (CHO) in which the viral long terminal repeat does not function efficiently.

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Year:  1990        PMID： 2352331      PMCID： PMC249623     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  41 in total

1.  Functional analysis of a retroviral host-range mutant: altered long terminal repeat sequences allow expression in embryonal carcinoma cells.

Authors:  F Hilberg; C Stocking; W Ostertag; M Grez
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

2.  Molecular events in the processing of recombinant type 1 pre-pro-transforming growth factor beta to the mature polypeptide.

Authors:  L E Gentry; M N Lioubin; A F Purchio; H Marquardt
Journal:  Mol Cell Biol       Date:  1988-10       Impact factor: 4.272

3.  Development of retrovirus vectors useful for expressing genes in cultured murine embryonal cells and hematopoietic cells in vivo.

Authors:  B C Guild; M H Finer; D E Housman; R C Mulligan
Journal:  J Virol       Date:  1988-10       Impact factor: 5.103

4.  Stably transmitted triple-promoter retroviral vectors and their use in transformation of primary mammalian cells.

Authors:  R W Overell; K E Weisser; D Cosman
Journal:  Mol Cell Biol       Date:  1988-04       Impact factor: 4.272

5.  Negative control region at the 5' end of murine leukemia virus long terminal repeats.

Authors:  J R Flanagan; A M Krieg; E E Max; A S Khan
Journal:  Mol Cell Biol       Date:  1989-02       Impact factor: 4.272

6.  Human purine nucleoside phosphorylase and adenosine deaminase: gene transfer into cultured cells and murine hematopoietic stem cells by using recombinant amphotropic retroviruses.

Authors:  R S McIvor; M J Johnson; A D Miller; S Pitts; S R Williams; D Valerio; D W Martin; I M Verma
Journal:  Mol Cell Biol       Date:  1987-02       Impact factor: 4.272

7.  Type 1 transforming growth factor beta: amplified expression and secretion of mature and precursor polypeptides in Chinese hamster ovary cells.

Authors:  L E Gentry; N R Webb; G J Lim; A M Brunner; J E Ranchalis; D R Twardzik; M N Lioubin; H Marquardt; A F Purchio
Journal:  Mol Cell Biol       Date:  1987-10       Impact factor: 4.272

8.  Gene expression from transcriptionally disabled retroviral vectors.

Authors:  J K Yee; J C Moores; D J Jolly; J A Wolff; J G Respess; T Friedmann
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

9.  Transforming growth factor beta 1 positively regulates its own expression in normal and transformed cells.

Authors:  E Van Obberghen-Schilling; N S Roche; K C Flanders; M B Sporn; A B Roberts
Journal:  J Biol Chem       Date:  1988-06-05       Impact factor: 5.157

10.  Retrovirus-mediated transfer of human adenosine deaminase gene sequences into cells in culture and into murine hematopoietic cells in vivo.

Authors:  D A Williams; S H Orkin; R C Mulligan
Journal:  Proc Natl Acad Sci U S A       Date:  1986-04       Impact factor: 11.205
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  1 in total

1.  Isolation of rsp-1, a novel cDNA capable of suppressing v-Ras transformation.

Authors:  M L Cutler; R H Bassin; L Zanoni; N Talbot
Journal:  Mol Cell Biol       Date:  1992-09       Impact factor: 4.272
  1 in total

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