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Literature DB >> 3037492

The SV40 termination region exhibits an altered helical DNA conformation.

Abstract

The DNA structure of a fragment containing the SV40 termination sequences was examined using gel mobility assays. The region is shown to contain a DNA bend as evidenced by an abnormal mobility that is progressively accentuated as the temperature is lowered. This represents the strongest example of DNA bending among the collection of SV40 fragments studied. The same fragment was shown previously to uniquely support hyper-stable nucleosome formation in vitro, suggesting a possible relationship between DNA bending and nucleosome stability.

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Year: 1987 PMID： 3037492 PMCID： PMC305970 DOI： 10.1093/nar/15.13.5433

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

25 in total

1. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors: T Maniatis; A Jeffrey; H van deSande
Journal: Biochemistry Date: 1975-08-26 Impact factor: 3.162

2. DNA bending induced by cruciform formation.

Authors: G W Gough; D M Lilley
Journal: Nature Date: 1985 Jan 10-18 Impact factor: 49.962

3. Sequence-induced DNA curvature at the bacteriophage lambda origin of replication.

Authors: K Zahn; F R Blattner
Journal: Nature Date: 1985 Oct 3-9 Impact factor: 49.962

4. On the sequence determinants and flexibility of the kinetoplast DNA fragment with abnormal gel electrophoretic mobilities.

Authors: S Diekmann; J C Wang
Journal: J Mol Biol Date: 1985-11-05 Impact factor: 5.469

5. Deletion analysis of a DNA sequence that positions itself precisely on the nucleosome core.

Authors: N Ramsay
Journal: J Mol Biol Date: 1986-05-05 Impact factor: 5.469

6. Visualization of the bent helix in kinetoplast DNA by electron microscopy.

Authors: J Griffith; M Bleyman; C A Rauch; P A Kitchin; P T Englund
Journal: Cell Date: 1986-08-29 Impact factor: 41.582

7. Physical characterization of a kinetoplast DNA fragment with unusual properties.

Authors: J C Marini; P N Effron; T C Goodman; C K Singleton; R D Wells; R M Wartell; P T Englund
Journal: J Biol Chem Date: 1984-07-25 Impact factor: 5.157

8. Mobility of DNA in gel electrophoresis.

Authors: O J Lumpkin
Journal: Biopolymers Date: 1982-11 Impact factor: 2.505

9. Competition for formation of nucleosomes on fragmented SV40 DNA: a hyperstable nucleosome forms on the termination region.

Authors: L G Poljak; J D Gralla
Journal: Biochemistry Date: 1987-01-13 Impact factor: 3.162

The SV40 termination region exhibits an altered helical DNA conformation.

1. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

2. DNA bending induced by cruciform formation.

3. Sequence-induced DNA curvature at the bacteriophage lambda origin of replication.

4. On the sequence determinants and flexibility of the kinetoplast DNA fragment with abnormal gel electrophoretic mobilities.

5. Deletion analysis of a DNA sequence that positions itself precisely on the nucleosome core.

6. Visualization of the bent helix in kinetoplast DNA by electron microscopy.

7. Physical characterization of a kinetoplast DNA fragment with unusual properties.

8. Mobility of DNA in gel electrophoresis.

9. Competition for formation of nucleosomes on fragmented SV40 DNA: a hyperstable nucleosome forms on the termination region.

10. A highly bent fragment of Crithidia fasciculata kinetoplast DNA.

1. A non-curved chicken lysozyme 5' matrix attachment site is 3' followed by a strongly curved DNA sequence.

2. Sequences that promote formation of catenated intertwines during termination of DNA replication.

3. Nucleosome assembly of simian virus 40 DNA in a mammalian cell extract.

4. Intrinsic curvature in the VP1 gene of SV40: comparison of solution and gel results.

5. Three regions in the DNA of plasmid pLS1 show sequence-directed static bending.

6. Role of global and local topology in the regulation of gene expression in Streptococcus pneumoniae.