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Literature DB >> 2160586

Nucleosome assembly of simian virus 40 DNA in a mammalian cell extract.

Abstract

We report here a mammalian cell-free system that can support chromatin assembly. Effective nucleosome assembly in HeLa cell extracts occurred at 125 to 200 mM KCl or potassium glutamate. At this physiological K+ ion concentration, two types of chromatin assembly were observed. The first was interfered with by Mg2+. Other cations such as Mn2+, Ca2+, Fe3+, and spermidine also inhibited this type of nucleosome assembly. The second type of assembly occurred in the presence of Mg2+ and at least equimolar ATP. However, even in the presence of ATP, excess Mg2+ inhibited assembly and promoted catenation of DNA; these effects could be circumvented by excess ATP, GTP, EDTA, or polyglutamic acid. The critical DNA concentration for optimum assembly in both pathways suggested a stoichiometric association of histones with DNA. The spacing of nucleosomes formed by both types of assembly on linear and circular DNA was reasonably regular, but chromatin assembled in the presence of ATP and Mg2+ was more stable.

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Year: 1990 PMID： 2160586 PMCID： PMC360648 DOI： 10.1128/mcb.10.6.2863-2873.1990

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

60 in total

1. Extracts of Drosophila embryos mediate chromatin assembly in vitro.

Authors: T Nelson; T S Hsieh; D Brutlag
Journal: Proc Natl Acad Sci U S A Date: 1979-11 Impact factor: 11.205

2. Acidic polypeptides can assemble both histones and chromatin in vitro at physiological ionic strength.

Authors: A Stein; J P Whitlock; M Bina
Journal: Proc Natl Acad Sci U S A Date: 1979-10 Impact factor: 11.205

3. Ribonucleic acid and other polyanions facilitate chromatin assembly in vitro.

Authors: T Nelson; R Wiegand; D Brutlag
Journal: Biochemistry Date: 1981-04-28 Impact factor: 3.162

4. Isolation of actively transcribed nucleosomes using immobilized HMG 14 and 17 and an analysis of alpha-globin chromatin.

Authors: S Weisbrod; H Weintraub
Journal: Cell Date: 1981-02 Impact factor: 41.582

5. DNA methylation in the human gamma delta beta-globin locus in erythroid and nonerythroid tissues.

Authors: L H van der Ploeg; R A Flavell
Journal: Cell Date: 1980-04 Impact factor: 41.582

6. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

7. Dephosphorylation and activation of Xenopus p34cdc2 protein kinase during the cell cycle.

Authors: J Gautier; T Matsukawa; P Nurse; J Maller
Journal: Nature Date: 1989-06-22 Impact factor: 49.962

8. Effects of pH on low-salt transition of chromatin core particles.

Authors: L J Libertini; E W Small
Journal: Biochemistry Date: 1982-07-06 Impact factor: 3.162

9. Phosphorylation states of different histone 1 subtypes and their relationship to chromatin functions during the HeLa S-3 cell cycle.

Authors: K Ajiro; T W Borun; L H Cohen
Journal: Biochemistry Date: 1981-03-17 Impact factor: 3.162

10. Asymmetry and polarity of nucleosomes in chicken erythrocyte chromatin.

Authors: S C Satchwell; A A Travers
Journal: EMBO J Date: 1989-01 Impact factor: 11.598

12 in total

1. In vitro chromatin assembly promoted by the Xenopus laevis S-150 cell-free extract is enhanced by treatment with RNase A.

Authors: J M Sekiguchi; E B Kmiec
Journal: Nucleic Acids Res Date: 1992-02-25 Impact factor: 16.971

10. Complementation of DNA repair in xeroderma pigmentosum group A cell extracts by a protein with affinity for damaged DNA.

Authors: P Robins; C J Jones; M Biggerstaff; T Lindahl; R D Wood
Journal: EMBO J Date: 1991-12 Impact factor: 11.598

Nucleosome assembly of simian virus 40 DNA in a mammalian cell extract.

1. Extracts of Drosophila embryos mediate chromatin assembly in vitro.

2. Acidic polypeptides can assemble both histones and chromatin in vitro at physiological ionic strength.

3. Ribonucleic acid and other polyanions facilitate chromatin assembly in vitro.

4. Isolation of actively transcribed nucleosomes using immobilized HMG 14 and 17 and an analysis of alpha-globin chromatin.

5. DNA methylation in the human gamma delta beta-globin locus in erythroid and nonerythroid tissues.

6. Sequencing end-labeled DNA with base-specific chemical cleavages.

7. Dephosphorylation and activation of Xenopus p34cdc2 protein kinase during the cell cycle.

8. Effects of pH on low-salt transition of chromatin core particles.

9. Phosphorylation states of different histone 1 subtypes and their relationship to chromatin functions during the HeLa S-3 cell cycle.

10. Asymmetry and polarity of nucleosomes in chicken erythrocyte chromatin.

1. In vitro chromatin assembly promoted by the Xenopus laevis S-150 cell-free extract is enhanced by treatment with RNase A.

2. Chromatin assembly in a yeast whole-cell extract.

3. Cell-free system for assembly of transcriptionally repressed chromatin from Drosophila embryos.

4. ATP dependent histone phosphorylation and nucleosome assembly in a human cell free extract.

5. Transcription complex disruption caused by a transition in chromatin structure.

6. Topoisomerase function during replication-independent chromatin assembly in yeast.

7. Assembly of nucleosomal DNA in a cell-free extract from wild-type and top1- strains of Ustilago maydis.

8. Postreplicative chromatin assembly by Drosophila and human chromatin assembly factor 1.

9. Transcriptionally competent chromatin assembled with exogenous histones in a yeast whole cell extract.

10. Complementation of DNA repair in xeroderma pigmentosum group A cell extracts by a protein with affinity for damaged DNA.