Literature DB >> 3035347

Replication of mini-F plasmid in vitro promoted by purified E protein.

K Muraiso, T Tokino, T Murotsu, K Matsubara.   

Abstract

An in vitro system for replication of mini-F plasmid DNA was constructed. This system consists of an ammonium sulfate fraction II (Fuller et al. 1981) from Escherichia coli extract, exogenously added purified E protein encoded by mini-F plasmid, and mini-F DNA in a closed circular form. Experiments with this system showed that the 217 bp DNA region which contains the A + T rich cluster and the four 19 bp direct repeats responsible for incB incompatibility is essential for mini-F DNA replication.

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Year:  1987        PMID： 3035347     DOI： 10.1007/BF00428895

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  17 in total

1.  Cloning, isolation, and characterization of replication regions of complex plasmid genomes.

Authors:  K Timmis; F Cabello; S N Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1975-06       Impact factor: 11.205

2.  A two-stage molecular model for control of mini-F replication.

Authors:  J D Trawick; B C Kline
Journal:  Plasmid       Date:  1985-01       Impact factor: 3.466

3.  Recombination between an Flac and a mini-FKmr plasmid deleted for an origin of replication.

Authors:  J Manis; B Kline
Journal:  Plasmid       Date:  1978-09       Impact factor: 3.466

4.  Enzymatic replication of the origin of the Escherichia coli chromosome.

Authors:  R S Fuller; J M Kaguni; A Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

5.  Role of nine repeating sequences of the mini-F genome for expression of F-specific incompatibility phenotype and copy number control.

Authors:  H Tsutsui; A Fujiyama; T Murotsu; K Matsubara
Journal:  J Bacteriol       Date:  1983-07       Impact factor: 3.490

6.  Restriction endonuclease mapping and mutagenesis of the F sex factor replication region.

Authors:  J J Manis; B C Kline
Journal:  Mol Gen Genet       Date:  1977-04-29

7.  An Mr 29000 protein is essential for mini-F maintenance in E. coli.

Authors:  L A Watson; S H Phua; P L Bergquist; H E Lane
Journal:  Gene       Date:  1982-09       Impact factor: 3.688

8.  Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid.

Authors:  M A Lovett; D R Helinski
Journal:  J Bacteriol       Date:  1976-08       Impact factor: 3.490

9.  Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid.

Authors:  M S Guyer; D Figurski; N Davidson
Journal:  J Bacteriol       Date:  1976-08       Impact factor: 3.490

10.  Two functions of the E protein are key elements in the plasmid F replication control system.

Authors:  L A Rokeach; L Søgaard-Andersen; S Molin
Journal:  J Bacteriol       Date:  1985-12       Impact factor: 3.490
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  3 in total

1.  Site-specific proteolysis of mini-F plasmid replication protein RepE destroys initiator function and generates an incompatibility substance.

Authors:  B C Kline; G S Sandhu; B W Eckloff; R A Aleff
Journal:  J Bacteriol       Date:  1992-05       Impact factor: 3.490

2.  Replication initiator protein RepE of mini-F plasmid: functional differentiation between monomers (initiator) and dimers (autogenous repressor).

Authors:  M Ishiai; C Wada; Y Kawasaki; T Yura
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-26       Impact factor: 11.205

3.  DNA-binding domain of the RepE initiator protein of mini-F plasmid: involvement of the carboxyl-terminal region.

Authors:  F Matsunaga; Y Kawasaki; M Ishiai; K Nishikawa; T Yura; C Wada
Journal:  J Bacteriol       Date:  1995-04       Impact factor: 3.490
  3 in total

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