| Literature DB >> 783125 |
M S Guyer, D Figurski, N Davidson.
Abstract
pML31, a plasmid chimera constructed to contain the replication genes of an Flac plasmid, has been studied by electron microscope methods. Heteroduplex analysis shows that the only F sequence present in pML31 is that with corrdinates 40.3-49.3F. This region has previously been identified as essential for plasmid maintenance. The sequence of pML31, which was derived originally from R6-5, carries the km gene(s) and an inverted duplication of a 1.0-kilobase sequence. On the basis of length measurements, the repeated sequence is different from IS1, IS2, IS3, and an inverted repeat associated with the km gene(s) of plasmid JR67.Entities:
Mesh:
Substances:
Year: 1976 PMID: 783125 PMCID: PMC233009 DOI: 10.1128/jb.127.2.988-997.1976
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490