Literature DB >> 3035078

Cytopathology, plaque assay, and heat inactivation of hepatitis A virus strain HM175.

D A Anderson.   

Abstract

Hepatitis A virus (HAV) strain HM175 derived after repeated subculture of persistently infected B-SC-1 cells caused a specific cytopathic effect upon acute infection of B-SC-1 cells. The virus formed visible plaques on B-SC-1 cell monolayers after 9 to 14 days of incubation at 34 degrees C, and virus can therefore be titrated by plaque assay. Virus could be re-isolated from plaques of infected cells, which allows the clonal isolation of HAV variants. The stability of a plaque-purified variant of HAV at elevated temperatures exceeded that of poliovirus type 1, but this variant is less stable than previously reported strains of HAV.

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Year:  1987        PMID： 3035078     DOI： 10.1002/jmv.1890220106

Source DB:  PubMed          Journal:  J Med Virol        ISSN： 0146-6615            Impact factor:   2.327


  18 in total

1.  Uncoating kinetics of hepatitis A virus virions and provirions.

Authors:  N E Bishop; D A Anderson
Journal:  J Virol       Date:  2000-04       Impact factor: 5.103

2.  Mutational characteristics in consecutive passage of rapidly replicating variants of hepatitis A virus strain H2 during cell culture adaptation.

Authors:  Ning-Zhu Hu; Yun-Zhang Hu; Hai-Jing Shi; Guo-Dong Liu; Su Qu
Journal:  World J Gastroenterol       Date:  2002-10       Impact factor: 5.742

3.  Antigenic and genetic variation in cytopathic hepatitis A virus variants arising during persistent infection: evidence for genetic recombination.

Authors:  S M Lemon; P C Murphy; P A Shields; L H Ping; S M Feinstone; T Cromeans; R W Jansen
Journal:  J Virol       Date:  1991-04       Impact factor: 5.103

Review 4.  Hepatology.

Authors:  P M Harrison; J Y Lau; R Williams
Journal:  Postgrad Med J       Date:  1991-08       Impact factor: 2.401

5.  A cytopathogenic, apoptosis-inducing variant of hepatitis A virus.

Authors:  K Brack; W Frings; A Dotzauer; A Vallbracht
Journal:  J Virol       Date:  1998-04       Impact factor: 5.103

6.  In situ hybridization for quantitative assay of infectious hepatitis A virus.

Authors:  J N Xi; M K Estes; T G Metcalf
Journal:  J Clin Microbiol       Date:  1989-05       Impact factor: 5.948

7.  Production of cytopathology in FRhK-4 cells by BS-C-1-passaged hepatitis A virus.

Authors:  A M Nasser; T G Metcalf
Journal:  Appl Environ Microbiol       Date:  1987-12       Impact factor: 4.792

8.  Morphological changes in HAV-infected Frp/3 cells and immunolocalization of HAAg.

Authors:  A Tinari; F M Ruggeri; M Divizia; A Panà; G Donelli
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

9.  Nucleotide sequence of wild-type hepatitis A virus GBM in comparison with two cell culture-adapted variants.

Authors:  J Graff; A Normann; S M Feinstone; B Flehmig
Journal:  J Virol       Date:  1994-01       Impact factor: 5.103

10.  A simple and rapid Hepatitis A Virus (HAV) titration assay based on antibiotic resistance of infected cells: evaluation of the HAV neutralization potency of human immune globulin preparations.

Authors:  Krishnamurthy Konduru; Maria Luisa Virata-Theimer; Mei-Ying W Yu; Gerardo G Kaplan
Journal:  Virol J       Date:  2008-12-18       Impact factor: 4.099
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