| Literature DB >> 3031589 |
D J Wozniak, D C Cram, C J Daniels, D R Galloway.
Abstract
We have previously reported the discovery and subsequent cloning of a regulatory gene, designated toxR, which appears to regulate the expression of the exotoxin A (ETA) structural gene toxA. Subsequent work by this laboratory has resulted in the subcloning of the toxR gene and its transfer to a high copy number plasmid (pGW28). Functional analysis of the toxR gene using a Tn5 insertion along with toxR deletions indicates that inactivation of toxR results in a dramatic reduction of ETA production. Nucleotide sequence analysis of pGW28 has revealed a 675 bp major open reading frame (225 codons) which could encode for a protein of 24,626 daltons. Using S1 nuclease mapping, the toxR RNA transcript has been shown to originate 20 bp upstream of the presumptive translation initiation codon. Experiments using a toxA specific probe have revealed the the toxR gene product appears to regulate the expression of ETA at the transcriptional level.Entities:
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Year: 1987 PMID： 3031589 PMCID： PMC340621 DOI： 10.1093/nar/15.5.2123
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 19.160