Gulcin Ercan1, Yuksel Altinel2, Onur Olgac Karagulle3, Hakan Yiğitbaş1, Nadir Adnan Hacım1, Serhat Meriç1, Nihat Buğdaycı4, Rumeysa Ilbar Tartar5, Burcu Biltekin6, Erkan Yavuz7, Osman Bilgin Gulcicek7, Ali Solmaz8, Atilla Çelik7. 1. MD. University of Health Sciences - Istanbul Bagcilar Training and Research Hospital - Department of General Surgery- Istanbul, Turkey. 2. MD, MSc. University of Health Sciences - Istanbul Bagcilar Training and Research Hospital - Department of General Surgery - Istanbul, Turkey. 3. MD. University of Health Sciences - Istanbul Training and Research Hospital - Department of General Surgery - Istanbul, Turkey. 4. MD. Sanliurfa Education and Research Hospital - Department of General Surgery - Sanliurfa Province Health Directorate - Sanliurfa, Turkey. 5. MD. University of Health Sciences - Sisli Hamidiye Etfal Training and Research Hospital - Department of General Surgery - Istanbul, Turkey. 6. PhD. Istanbul Atlas University - Faculty of Medicine - Department of Histology and Embryology - Istanbul, Turkey. 7. MD, Associate Professor. University of Health Sciences - Istanbul Bagcilar Training and Research Hospital - Department of General Surgery - Istanbul, Turkey. 8. MD, PhD, Associate Professor. Camlica Erdem Hospital - Department of General Surgery - Istanbul, Turkey.
Abstract
PURPOSE: To investigate the efficacy of cordycepin, an adenosine analogue, on prevention of esophageal damage and stricture formation due to esophageal caustic burns in rat model comparing with prednisolone. METHODS: Caustic esophageal burn was introduced by 37.5% of NaOH to distal esophagus. Thirty-two Wistar albino rats were divided in four groups: sham rats undergone laparotomy, treated with 0.9% NaCl; control rats injured with NaOH without cordycepin treatment; cordycepin group injured with NaOH, treated with 20 mg/kg cordycepin; prednisolone group injured with NaOH, treated with 1 mg/kg prednisolone for 28 days. Efficacy was assessed by histopathological and immunohistochemical analysis of esophageal tissues. RESULTS: Cordycepin treatment significantly decreased inflammation, granulation tissue and fibrous tissue formation and prevented formation of esophageal strictures shown by histopathological damage score and stenosis indexes compared to control group (p < 0.01). These effects are relatively more substantial than prednisolone, probably based on attenuation of elevation of proinflammatory cytokines hypoxia-inducible factor 1-alpha (HIF-1?), tumor necrosis factor alpha (TNF-?), proliferative and fibrotic factor fibroblast growth factor 2 (FGF2) and angiogenic factor vascular endothelial growth factor A (VEGFA) (p < 0.05). CONCLUSIONS: The findings suggest that cordycepin has a complex multifactorial healing process in alkali-burned tissue, more successful than prednisolone in preventing the formation of esophageal strictures and may be used as a therapeutic agent in the acute phase of esophageal alkali-burn.
PURPOSE: To investigate the efficacy of cordycepin, an adenosine analogue, on prevention of esophageal damage and stricture formation due to esophageal caustic burns in rat model comparing with prednisolone. METHODS: Caustic esophageal burn was introduced by 37.5% of NaOH to distal esophagus. Thirty-two Wistar albino rats were divided in four groups: sham rats undergone laparotomy, treated with 0.9% NaCl; control rats injured with NaOH without cordycepin treatment; cordycepin group injured with NaOH, treated with 20 mg/kg cordycepin; prednisolone group injured with NaOH, treated with 1 mg/kg prednisolone for 28 days. Efficacy was assessed by histopathological and immunohistochemical analysis of esophageal tissues. RESULTS:Cordycepin treatment significantly decreased inflammation, granulation tissue and fibrous tissue formation and prevented formation of esophageal strictures shown by histopathological damage score and stenosis indexes compared to control group (p < 0.01). These effects are relatively more substantial than prednisolone, probably based on attenuation of elevation of proinflammatory cytokines hypoxia-inducible factor 1-alpha (HIF-1?), tumor necrosis factor alpha (TNF-?), proliferative and fibrotic factor fibroblast growth factor 2 (FGF2) and angiogenic factor vascular endothelial growth factor A (VEGFA) (p < 0.05). CONCLUSIONS: The findings suggest that cordycepin has a complex multifactorial healing process in alkali-burned tissue, more successful than prednisolone in preventing the formation of esophageal strictures and may be used as a therapeutic agent in the acute phase of esophageal alkali-burn.