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Literature DB >> 3029271

A murine hybridoma with large cytoplasmic inclusions of kappa light chains.

Abstract

A murine hybridoma cell line has been established that consistently forms large cytoplasmic inclusions. These structures bind antibody specific for mouse kappa L chain when stained in situ. SDS-PAGE analysis of isolated inclusion bodies produce a single protein band of approximately 26,000 Mr that reacts with anti-kappa antibody when transferred to nitrocellulose. No carbohydrate was detected in association with the purified protein. These data are consistent with the intracellular retention and deposition of complete kappa L chain protein.

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Year: 1987 PMID： 3029271 PMCID： PMC2188503 DOI： 10.1084/jem.165.2.578

Source DB: PubMed Journal: J Exp Med ISSN： 0022-1007 Impact factor: 14.307

14 in total

1. A sensitive fluorescent method for the detection of glycoproteins in polyacrylamide gels.

Authors: A E Eckhardt; C E Hayes; I J Goldstein
Journal: Anal Biochem Date: 1976-05-21 Impact factor: 3.365

2. Intracellular transport blockade caused by disruption of the disulfide bridge in the third external domain of major histocompatibility complex class I antigen.

Authors: J Miyazaki; E Appella; K Ozato
Journal: Proc Natl Acad Sci U S A Date: 1986-02 Impact factor: 11.205

9. Primary structure of the variable part of an amyloidogenic Bence-Jones Protein (Mev.). An unusual insertion in the third hypervariable region of a human kappa-immunoglobulin light chain.

Authors: M Eulitz; R P Linke
Journal: Hoppe Seylers Z Physiol Chem Date: 1982-11

10. Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy.

Authors: M H Heggeness; J F Ash
Journal: J Cell Biol Date: 1977-06 Impact factor: 10.539

2 in total

1. Intermolecular interactions involving an acidic patch on immunoglobulin variable domain and the γ2 constant region mediate crystalline inclusion body formation in the endoplasmic reticulum.

Authors: Haruki Hasegawa; Mei Geng; Randal R Ketchem; Ling Liu; Kevin Graham; Frederick Jacobsen
Journal: Cell Logist Date: 2017-08-08

2. Aggregates, crystals, gels, and amyloids: intracellular and extracellular phenotypes at the crossroads of immunoglobulin physicochemical property and cell physiology.

Authors: Haruki Hasegawa
Journal: Int J Cell Biol Date: 2013-03-05

2 in total

A murine hybridoma with large cytoplasmic inclusions of kappa light chains.

1. A sensitive fluorescent method for the detection of glycoproteins in polyacrylamide gels.

2. Intracellular transport blockade caused by disruption of the disulfide bridge in the third external domain of major histocompatibility complex class I antigen.

3. Intracellular IgA immunoglobulin crystals in chronic lymphocytic leukaemia.

4. Amyloid fibril proteins: proof of homology with immunoglobulin light chains by sequence analyses.

5. Creation of "amyloid" fibrils from Bence Jones proteins in vitro.

6. Needle-shaped inclusions in plasma cells in a patient with hypogammaglobulinemia.

7. Primary gastric plasmacytoma with massive intracytoplasmic crystalline inclusions: a case report.

8. Studies of the mechanism of tunicamycin in hibition of IgA and IgE secretion by plasma cells.

9. Primary structure of the variable part of an amyloidogenic Bence-Jones Protein (Mev.). An unusual insertion in the third hypervariable region of a human kappa-immunoglobulin light chain.

10. Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy.

1. Intermolecular interactions involving an acidic patch on immunoglobulin variable domain and the γ2 constant region mediate crystalline inclusion body formation in the endoplasmic reticulum.

2. Aggregates, crystals, gels, and amyloids: intracellular and extracellular phenotypes at the crossroads of immunoglobulin physicochemical property and cell physiology.