Literature DB >> 326797

Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy.

M H Heggeness, J F Ash.   

Abstract

A new indirect method for fluorescence localization of proteins making use of the avidin-biotin complex is described. We have prepared both a biotin-modified rabbit heavy meromyosin (BHMM) and a biotin-modified antibody to a smooth muscle myosin. After fixation, cells can be treated with either BHMM, which binds to actin, or the biotinyl antibody, which binds to myosin. In a second step the cell are treated with a fluorescent derivative of avidin (Fl-avidin) which binds to the biotinyl proteins and thus indirectly reveals the location of the cellular action or myosin.

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Year:  1977        PMID： 326797      PMCID： PMC2111432          DOI： 10.1083/jcb.73.3.783

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  13 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Avidin.

Authors:  N M Green
Journal:  Adv Protein Chem       Date:  1975

3.  Reversion from transformed to normal phenotype by inhibition of protein synthesis in rat kidney cells infected with a temperature-sensitive mutant of Rous sarcoma virus.

Authors:  J F Ash; P K Vogt; S J Singer
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

4.  Antibody to myosin: the specific visualization of myosin-containing filaments in nonmuscle cells.

Authors:  K Weber; U Groeschel-Stewart
Journal:  Proc Natl Acad Sci U S A       Date:  1974-11       Impact factor: 11.205

5.  Determination of protein: a modification of the Lowry method that gives a linear photometric response.

Authors:  E F Hartree
Journal:  Anal Biochem       Date:  1972-08       Impact factor: 3.365

6.  The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.

Authors:  J A Spudich; S Watt
Journal:  J Biol Chem       Date:  1971-08-10       Impact factor: 5.157

7.  Substructure of the myosin molecule. I. Subfragments of myosin by enzymic degradation.

Authors:  S Lowey; H S Slayter; A G Weeds; H Baker
Journal:  J Mol Biol       Date:  1969-05-28       Impact factor: 5.469

8.  Filamin, a new high-molecular-weight protein found in smooth muscle and non-muscle cells.

Authors:  K Wang; J F Ash; S J Singer
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205

9.  Use of the avidin-biotin complex for specific staining of biological membranes in electron microscopy.

Authors:  H Heitzmann; F M Richards
Journal:  Proc Natl Acad Sci U S A       Date:  1974-09       Impact factor: 11.205

10.  Actin antibody: the specific visualization of actin filaments in non-muscle cells.

Authors:  E Lazarides; K Weber
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205
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  40 in total

1.  Production of human monoclonal anti-basement membrane zone (BMZ) antibodies from a patient with bullous pemphigoid (BP) by Epstein-Barr virus transformation. Analyses of the heterogeneity of anti-BMZ antibodies in BP sera using them.

Authors:  T Sugi; T Hashimoto; T Hibi; T Nishikawa
Journal:  J Clin Invest       Date:  1989-10       Impact factor: 14.808

2.  Antibody-induced linkages of plasma membrane proteins to intracellular actomyosin-containing filaments in cultured fibroblasts.

Authors:  J F Ash; D Louvard; S J Singer
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

3.  Monovalent Strep-Tactin for strong and site-specific tethering in nanospectroscopy.

Authors:  Fabian Baumann; Magnus S Bauer; Lukas F Milles; Alexander Alexandrovich; Hermann E Gaub; Diana A Pippig
Journal:  Nat Nanotechnol       Date:  2015-10-12       Impact factor: 39.213

4.  Rubella virus antigens: localization of epitopes involved in hemagglutination and neutralization by using monoclonal antibodies.

Authors:  K Y Green; P H Dorsett
Journal:  J Virol       Date:  1986-03       Impact factor: 5.103

5.  Distinguishing the antigen specificities of pemphigus vulgaris and pemphigus foliaceus using the biotin-avidin immunofluorescence method.

Authors:  T Muramatsu; K Sakamoto; S Ikeda; H Ogawa
Journal:  Arch Dermatol Res       Date:  1987       Impact factor: 3.017

6.  Soluble HLA class I antigens in sera of patients with chronic hepatitis.

Authors:  K Sakaguchi; N Koide; T Takenami; H Matsushima; H Takabatake; S Ferrone; T Tsuji
Journal:  Gastroenterol Jpn       Date:  1992-04

7.  Paradoxical effect of pilus expression on binding of antibodies by Haemophilus influenzae.

Authors:  J R Gilsdorf; M Tucci; L J Forney; W Watson; C F Marrs; E J Hansen
Journal:  Infect Immun       Date:  1993-08       Impact factor: 3.441

8.  Interaction of monoclonal antibodies with the IgE-receptor on rat mast cells and rat basophilic leukemia (RBL) cells.

Authors:  G Micklefield; W König; P Pfeiffer; A Bohn
Journal:  Agents Actions       Date:  1986-03

9.  Intracellular distributions of mechanochemical proteins in cultured fibroblasts.

Authors:  M H Heggeness; K Wang; S J Singer
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

10.  Methylamine-treated low density lipoproteins elicit different responses in HepG2 cells and macrophages.

Authors:  E Koren; N Dashti; P R Wilson; D M Lee
Journal:  Mol Cell Biochem       Date:  1993-07-07       Impact factor: 3.396
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