| Literature DB >> 30291453 |
Fangmei Zhang1,2, Pinyi Ma3, Xinyu Deng1, Ying Sun1, Xinghua Wang1, Daqian Song4.
Abstract
Glutathione-capped water-soluble CuInS/ZnS quantum dots (QDs) were prepared by a microwave-assisted method. Their fluorescence, with excitation/emission peaks at 380/570 nm, is found to be quenched by hydrogen peroxide (H2O2) that is produced by the uricase catalyzed oxidation of uric acid (UA) and oxygen. The findings are used in a quenchometric method for the determination of UA. The effects of different ligands on the QDs, of pH value, buffers, enzyme ratio and reaction time were optimized. The detection limit for UA is 50 nM which is lower than other QD-based method, and the detection ranges extends from 0.25-4.0 μM. The assay is simple and sensitive, and no further modification of the QDs is required. Graphical abstract ᅟ.Entities:
Keywords: Fluorescent probe; Human urine and serum; Microwave-based synthesis; Nanoparticle synthesis; Uric acid determination
Year: 2018 PMID: 30291453 DOI: 10.1007/s00604-018-3030-0
Source DB: PubMed Journal: Mikrochim Acta ISSN: 0026-3672 Impact factor: 5.833