| Literature DB >> 25113659 |
Nur Ellina Azmi1, Noor Izaanin Ramli2, Jaafar Abdullah3, Mohammad Azmi Abdul Hamid4, Hamidah Sidek2, Samsulida Abd Rahman5, Nurhayati Ariffin2, Nor Azah Yusof6.
Abstract
A novel optical detection system consisting of combination of uricase/HRP-CdS quantum dots (QDs) for the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horseradish peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence, which was proportional to uric acid concentration. The system demonstrated sufficient activity of uricase and HRP at a ratio of 5U:5U and pH 7.0. The linearity of the system toward uric acid was in the concentration range of 125-1000 µM with detection limit of 125 µM.Entities:
Keywords: Enzyme; Fluorescence; Quantum dots; Quenching; Uric acid
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Year: 2014 PMID： 25113659 DOI： 10.1016/j.bios.2014.07.056
Source DB: PubMed Journal: Biosens Bioelectron ISSN： 0956-5663 Impact factor: 10.618