Literature DB >> 30283810

In vitro Enzymatic Assays of Histone Decrotonylation on Recombinant Histones.

Rachel Fellows1, Patrick Varga-Weisz1,2.   

Abstract

Class I histone deacetylases (HDACs) are efficient histone decrotonylases, broadening the enzymatic spectrum of these important (epi-)genome regulators and drug targets. Here, we describe an in vitro approach to assaying class I HDACs with different acyl-histone substrates, including crotonylated histones and expand this to examine the effect of inhibitors and estimate kinetic constants.

Entities:  

Keywords:  Acetylation; Butyrylation; Chromatin; Crotonylation; Enzyme; Histone deacetylase; Kinetic

Year:  2018        PMID： 30283810      PMCID： PMC6166789          DOI： 10.21769/BioProtoc.2924

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  10 in total

Review 1.  Recent progress in the development of assays suited for histone deacetylase inhibitor screening.

Authors:  Dennis Wegener; Christian Hildmann; Andreas Schwienhorst
Journal:  Mol Genet Metab       Date:  2003 Sep-Oct       Impact factor: 4.797

2.  Improved fluorogenic histone deacetylase assay for high-throughput-screening applications.

Authors:  Dennis Wegener; Christian Hildmann; Daniel Riester; Andreas Schwienhorst
Journal:  Anal Biochem       Date:  2003-10-15       Impact factor: 3.365

3.  A fluorogenic histone deacetylase assay well suited for high-throughput activity screening.

Authors:  Dennis Wegener; Frank Wirsching; Daniel Riester; Andreas Schwienhorst
Journal:  Chem Biol       Date:  2003-01

Review 4.  Regulation of chromatin by histone modifications.

Authors:  Andrew J Bannister; Tony Kouzarides
Journal:  Cell Res       Date:  2011-02-15       Impact factor: 25.617

5.  Class I histone deacetylases are major histone decrotonylases: evidence for critical and broad function of histone crotonylation in transcription.

Authors:  Wei Wei; Xiaoguang Liu; Jiwei Chen; Shennan Gao; Lu Lu; Huifang Zhang; Guangjin Ding; Zhiqiang Wang; Zhongzhou Chen; Tieliu Shi; Jiwen Li; Jianjun Yu; Jiemin Wong
Journal:  Cell Res       Date:  2017-05-12       Impact factor: 25.617

Review 6.  Histone Post-Translational Modifications and Nucleosome Organisation in Transcriptional Regulation: Some Open Questions.

Authors:  Josefa Castillo; Gerardo López-Rodas; Luis Franco
Journal:  Adv Exp Med Biol       Date:  2017       Impact factor: 2.622

7.  Intracellular crotonyl-CoA stimulates transcription through p300-catalyzed histone crotonylation.

Authors:  Benjamin R Sabari; Zhanyun Tang; He Huang; Vladimir Yong-Gonzalez; Henrik Molina; Ha Eun Kong; Lunzhi Dai; Miho Shimada; Justin R Cross; Yingming Zhao; Robert G Roeder; C David Allis
Journal:  Mol Cell       Date:  2015-03-26       Impact factor: 17.970

8.  A histone mutant reproduces the phenotype caused by loss of histone-modifying factor Polycomb.

Authors:  Ana Raquel Pengelly; Ömer Copur; Herbert Jäckle; Alf Herzig; Jürg Müller
Journal:  Science       Date:  2013-02-08       Impact factor: 47.728

9.  Microbiota derived short chain fatty acids promote histone crotonylation in the colon through histone deacetylases.

Authors:  Rachel Fellows; Jérémy Denizot; Claudia Stellato; Alessandro Cuomo; Payal Jain; Elena Stoyanova; Szabina Balázsi; Zoltán Hajnády; Anke Liebert; Juri Kazakevych; Hector Blackburn; Renan Oliveira Corrêa; José Luís Fachi; Fabio Takeo Sato; Willian R Ribeiro; Caroline Marcantonio Ferreira; Hélène Perée; Mariangela Spagnuolo; Raphaël Mattiuz; Csaba Matolcsi; Joana Guedes; Jonathan Clark; Marc Veldhoen; Tiziana Bonaldi; Marco Aurélio Ramirez Vinolo; Patrick Varga-Weisz
Journal:  Nat Commun       Date:  2018-01-09       Impact factor: 14.919

Review 10.  Metabolic regulation of gene expression through histone acylations.

Authors:  Benjamin R Sabari; Di Zhang; C David Allis; Yingming Zhao
Journal:  Nat Rev Mol Cell Biol       Date:  2016-12-07       Impact factor: 94.444
  10 in total

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