| Literature DB >> 3027952 |
S Edwards, D Chasey, P Napthine, J Banks, C Hewitt-Taylor, M P Cranage.
Abstract
Three techniques for the detection of rotavirus in faecal samples from calves with neonatal gastroenteritis were compared. A preliminary study indicated that reverse passive haemagglutination (RPHA) was at least as sensitive as the enzyme-linked immunosorbent assay (ELISA). These two immunoassays were compared with the detection of viral RNA by polyacrylamide gel electrophoresis (PAGE) on 209 field samples. Of the 77 samples in which at least one test gave a positive result, 69 were positive by both RPHA and PAGE, but only 49 were also positive by ELISA, indicating a lower sensitivity for the latter test. The overall agreement between RPHA and PAGE was 96%. The reasons for the discrepancies between the tests are discussed.Entities:
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Year: 1987 PMID： 3027952 PMCID： PMC7117104 DOI： 10.1016/0378-1135(87)90094-0
Source DB: PubMed Journal: Vet Microbiol ISSN： 0378-1135 Impact factor: 3.293