Literature DB >> 6169771

Eggs: conveniently packaged antibodies. Methods for purification of yolk IgG.

J C Jensenius, I Andersen, J Hau, M Crone, C Koch.   

Abstract

Eggs from immunized chickens may provide a convenient and inexpensive source of antibodies. We describe two simple and efficient methods for purification of IgG from yolk. The antibody is rendered useful for most currently employed immunological techniques. Amounts of antibody corresponding to almost half a litre of antiserum may be recovered from a chicken in one month.

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Year:  1981        PMID: 6169771     DOI: 10.1016/0022-1759(81)90333-1

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  47 in total

1.  Methemoglobin is a supplement for in vitro culture of human nasopharyngeal epithelial cells transformed by human papillomavirus type 16 DNA.

Authors:  W N Wen
Journal:  In Vitro Cell Dev Biol Anim       Date:  2001 Nov-Dec       Impact factor: 2.416

2.  Identification and characterization of an 18-kilodalton, VAMP-like protein in suspension-cultured carrot cells.

Authors:  M Gasparian; M Pusterla; B Baldan; P M Downey; O Rossetto; P P de Laureto; F Filippini; M Terzi; F Lo Schiavo
Journal:  Plant Physiol       Date:  2000-01       Impact factor: 8.340

3.  Hen egg immunoglobulin Y in colloidal gold agglutination assay: comparison with rabbit immunoglobulin G.

Authors:  Vardan K Gasparyan
Journal:  J Clin Lab Anal       Date:  2005       Impact factor: 2.352

4.  The 32-Kilodalton Vegetative Storage Protein of Salix microstachya Turz : Characterization and Immunolocalization.

Authors:  S Wetzel; J S Greenwood
Journal:  Plant Physiol       Date:  1991-10       Impact factor: 8.340

5.  Mucosal priming of T-lymphocyte responses to fed protein antigens using cholera toxin as an adjuvant.

Authors:  C J Clarke; A D Wilson; N A Williams; C R Stokes
Journal:  Immunology       Date:  1991-03       Impact factor: 7.397

6.  The chicken leukocyte receptor complex encodes a primordial, activating, high-affinity IgY Fc receptor.

Authors:  Birgit C Viertlboeck; Sonja Schweinsberg; Matthias A Hanczaruk; Ramona Schmitt; Louis Du Pasquier; Friedrich W Herberg; Thomas W Göbel
Journal:  Proc Natl Acad Sci U S A       Date:  2007-07-02       Impact factor: 11.205

7.  Development of an enzyme-linked immunosorbent assay for the detection of ciguatoxin in fish tissue using chicken immunoglobulin Y.

Authors:  Cara Empey Campora; Yoshitsugi Hokama; Kenichi Yabusaki; Minoru Isobe
Journal:  J Clin Lab Anal       Date:  2008       Impact factor: 2.352

8.  Identification of heat shock protein hsp70 homologues in chloroplasts.

Authors:  J S Marshall; A E DeRocher; K Keegstra; E Vierling
Journal:  Proc Natl Acad Sci U S A       Date:  1990-01       Impact factor: 11.205

9.  Altered expression of small proteoglycans, collagen, and transforming growth factor-beta 1 in developing bleomycin-induced pulmonary fibrosis in rats.

Authors:  G Westergren-Thorsson; J Hernnäs; B Särnstrand; A Oldberg; D Heinegård; A Malmström
Journal:  J Clin Invest       Date:  1993-08       Impact factor: 14.808

10.  Spontaneous development of anti-hepatitis B virus envelope (anti-idiotypic) antibodies in animals immunized with human liver endonexin II or with the F(ab')2 fragment of anti-human liver endonexin II immunoglobulin G: evidence for a receptor-ligand-like relationship between small hepatitis B surface antigen and endonexin II.

Authors:  K Hertogs; E Depla; T Crabbé; W De Bruin; W Leenders; H Moshage; S H Yap
Journal:  J Virol       Date:  1994-03       Impact factor: 5.103

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